2x1l

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[[Image:2x1l.png|left|200px]]
 
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{{STRUCTURE_2x1l| PDB=2x1l | SCENE= }}
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==Crystal structure of Mycobacterium smegmatis methionyl-tRNA synthetase in complex with methionine and adenosine==
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<StructureSection load='2x1l' size='340' side='right'caption='[[2x1l]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x1l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X1L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>, <scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1l OCA], [https://pdbe.org/2x1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x1l RCSB], [https://www.ebi.ac.uk/pdbsum/2x1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x1l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0R3E2_MYCS2 A0R3E2_MYCS2] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).[SAAS:SAAS023457_004_015657][HAMAP-Rule:MF_01228]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x1/2x1l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x1l ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two structures of monomeric methionyl-tRNA synthetase, from Mycobacterium smegmatis, in complex with the ligands methionine/adenosine and methionine, were analyzed by X-ray crystallography at 2.3 A and at 2.8 A, respectively. The structures demonstrated the flexibility of the multidomain enzyme. A new conformation of the structure was identified in which the connective peptide domain bound more closely to the catalytic domain than described previously. The KMSKS(301-305) loop in our structures was in an open and inactive conformation that differed from previous structures by a rotation of the loop of about 90 degrees around hinges located at Asn297 and Val310. The binding of adenosine to the methionyl-tRNA synthetase methionine complex caused a shift in the KMSKS domain that brought it closer to the catalytic domain. The potential use of the adenosine-binding site for inhibitor binding was evaluated and a potential binding site for a specific allosteric inhibitor was identified.
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===CRYSTAL STRUCTURE OF MYCOBACTERIUM SMEGMATIS METHIONYL-TRNA SYNTHETASE IN COMPLEX WITH METHIONINE AND ADENOSINE===
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Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase.,Ingvarsson H, Unge T FEBS J. 2010 Aug 26. PMID:20796028<ref>PMID:20796028</ref>
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{{ABSTRACT_PUBMED_20796028}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2x1l" style="background-color:#fffaf0;"></div>
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[[2x1l]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1L OCA].
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==See Also==
==See Also==
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*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]]
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020796028</ref><ref group="xtra">PMID:019478446</ref><references group="xtra"/>
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__TOC__
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[[Category: Methionine--tRNA ligase]]
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</StructureSection>
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[[Category: Mycobacterium smegmatis]]
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[[Category: Large Structures]]
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[[Category: Ingvarsson, H.]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: Jones, T A.]]
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[[Category: Ingvarsson H]]
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[[Category: Unge, T.]]
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[[Category: Jones TA]]
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[[Category: Aminoacyl-trna synthetase]]
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[[Category: Unge T]]
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[[Category: Ligase]]
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[[Category: Nucleotide-binding]]
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[[Category: Protein biosynthesis]]
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Current revision

Crystal structure of Mycobacterium smegmatis methionyl-tRNA synthetase in complex with methionine and adenosine

PDB ID 2x1l

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