1l2q
From Proteopedia
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| - | [[Image:1l2q.jpg|left|200px]]<br /><applet load="1l2q" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1l2q, resolution 1.70Å" /> | ||
| - | '''Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)'''<br /> | ||
| - | == | + | ==Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)== |
| + | <StructureSection load='1l2q' size='340' side='right'caption='[[1l2q]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1l2q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=XPL:N~6~-[(3R,5R)-5-AMINO-3-METHYL-D-PROLYL]-L-LYSINE'>XPL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2q OCA], [https://pdbe.org/1l2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2q RCSB], [https://www.ebi.ac.uk/pdbsum/1l2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2q ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MTMB1_METBA MTMB1_METBA] Catalyzes the transfer of the methyl group from monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).<ref>PMID:9642198</ref> <ref>PMID:9195968</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. | Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. | ||
| - | + | A new UAG-encoded residue in the structure of a methanogen methyltransferase.,Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK Science. 2002 May 24;296(5572):1462-6. PMID:12029132<ref>PMID:12029132</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1l2q" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Monomethylamine methyltransferase|Monomethylamine methyltransferase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Methanosarcina barkeri]] | ||
| + | [[Category: Chan MK]] | ||
| + | [[Category: Ferguson TK]] | ||
| + | [[Category: Gong W]] | ||
| + | [[Category: Hao B]] | ||
| + | [[Category: James CM]] | ||
| + | [[Category: Krzycki JA]] | ||
Current revision
Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)
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