2l23

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[[Image:2l23.png|left|200px]]
 
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{{STRUCTURE_2l23| PDB=2l23 | SCENE= }}
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==NMR structure of the ACID (ACtivator Interacting Domain) of the human mediator Med25 protein==
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<StructureSection load='2l23' size='340' side='right'caption='[[2l23]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2l23]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L23 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l23 OCA], [https://pdbe.org/2l23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l23 RCSB], [https://www.ebi.ac.uk/pdbsum/2l23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l23 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/MED25_HUMAN MED25_HUMAN] Charcot-Marie-Tooth disease type 2B2. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:19290556</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/MED25_HUMAN MED25_HUMAN] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.<ref>PMID:14657022</ref> <ref>PMID:14983011</ref> <ref>PMID:17641689</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MED25 (ARC92/ACID1) is a 747 residues subunit specific to higher eukaryote Mediator complex, an essential component of the RNA polymerase II general transcriptional machinery. MED25 is a target of the Herpes simplex virus transactivator protein VP16. MED25 interacts with VP16 through a central MED25 PTOV (Prostate tumour overexpressed)/ACID (Activator interacting domain) domain of unknown structure. As a first step towards understanding the mechanism of recruitment of transactivation domains by MED25, we report here the NMR structure of the MED25 ACID domain. The domain architecture consists of a closed beta-barrel with seven strands (Beta1-Beta7) and three alpha-helices (H1-H3), an architecture showing similarities to that of the SPOC (Spen paralog and ortholog C-terminal domain) domain-like superfamily. Preliminary NMR chemical shift mapping showed that VP16 H2 (VP16C) interacts with MED25 ACID through one face of the beta-barrel, defined by strands B4-B7-B6.
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===NMR structure of the ACID (ACtivator Interacting Domain) of the human mediator Med25 protein===
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NMR structure of the human Mediator MED25 ACID domain.,Bontems F, Verger A, Dewitte F, Lens Z, Baert JL, Ferreira E, Launoit YD, Sizun C, Guittet E, Villeret V, Monte D J Struct Biol. 2010 Oct 23. PMID:20974256<ref>PMID:20974256</ref>
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{{ABSTRACT_PUBMED_20974256}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2l23" style="background-color:#fffaf0;"></div>
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[[2l23]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L23 OCA].
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==See Also==
==See Also==
*[[Mediator|Mediator]]
*[[Mediator|Mediator]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020974256</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Bontems, F.]]
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[[Category: Large Structures]]
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[[Category: Dewitte, F.]]
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[[Category: Bontems F]]
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[[Category: Monte, D.]]
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[[Category: Dewitte F]]
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[[Category: Villeret, V.]]
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[[Category: Monte D]]
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[[Category: Human mediator complex]]
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[[Category: Villeret V]]
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[[Category: Pancreatic tumor overexpressed domain]]
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[[Category: Transcription]]
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Current revision

NMR structure of the ACID (ACtivator Interacting Domain) of the human mediator Med25 protein

PDB ID 2l23

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