3mtn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:01, 6 September 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3mtn.png|left|200px]]
 
-
{{STRUCTURE_3mtn| PDB=3mtn | SCENE= }}
+
==Usp21 in complex with a ubiquitin-based, USP21-specific inhibitor==
 +
<StructureSection load='3mtn' size='340' side='right'caption='[[3mtn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3mtn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MTN FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mtn OCA], [https://pdbe.org/3mtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mtn RCSB], [https://www.ebi.ac.uk/pdbsum/3mtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mtn ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/UBP21_HUMAN UBP21_HUMAN] Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.<ref>PMID:10799498</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/3mtn_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mtn ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The ubiquitin system regulates virtually all aspects of cellular function. We report a method to target the myriad enzymes that govern ubiquitination of protein substrates. We used massively diverse combinatorial libraries of ubiquitin variants to develop inhibitors of four deubiquitinases (DUBs) and analyzed the DUB-inhibitor complexes with crystallography. We extended the selection strategy to the ubiquitin conjugating (E2) and ubiquitin ligase (E3) enzymes and found that ubiquitin variants can also enhance enzyme activity. Last, we showed that ubiquitin variants can bind selectively to ubiquitin-binding domains. Ubiquitin variants exhibit selective function in cells and thus enable orthogonal modulation of specific enzymatic steps in the ubiquitin system.
-
===Usp21 in complex with a ubiquitin-based, USP21-specific inhibitor===
+
A strategy for modulation of enzymes in the ubiquitin system.,Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS Science. 2013 Feb 1;339(6119):590-5. doi: 10.1126/science.1230161. Epub 2013 Jan , 3. PMID:23287719<ref>PMID:23287719</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
[[3mtn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MTN OCA].
+
<div class="pdbe-citations 3mtn" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
-
*[[Ubiquitin|Ubiquitin]]
+
*[[Thioesterase 3D structures|Thioesterase 3D structures]]
 +
*[[3D structures of ubiquitin|3D structures of ubiquitin]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Ubiquitin thiolesterase]]
+
[[Category: Large Structures]]
-
[[Category: Arrowsmith, C H.]]
+
[[Category: Arrowsmith CH]]
-
[[Category: Avvakumov, G V.]]
+
[[Category: Avvakumov GV]]
-
[[Category: Bochkarev, A.]]
+
[[Category: Bochkarev A]]
-
[[Category: Bountra, C.]]
+
[[Category: Bountra C]]
-
[[Category: Dhe-Paganon, S.]]
+
[[Category: Dhe-Paganon S]]
-
[[Category: Edwards, A M.]]
+
[[Category: Edwards AM]]
-
[[Category: Ernst, A.]]
+
[[Category: Ernst A]]
-
[[Category: Li, Y.]]
+
[[Category: Li Y]]
-
[[Category: SGC, Structural Genomics Consortium.]]
+
[[Category: Sidhu S]]
-
[[Category: Sidhu, S.]]
+
[[Category: Walker JR]]
-
[[Category: Walker, J R.]]
+
[[Category: Weigelt J]]
-
[[Category: Weigelt, J.]]
+
[[Category: Xue S]]
-
[[Category: Xue, S.]]
+
-
[[Category: Activator]]
+
-
[[Category: Chromatin regulator]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Inhibitor]]
+
-
[[Category: Isopeptide bond]]
+
-
[[Category: Nucleus]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Protease]]
+
-
[[Category: Sgc]]
+
-
[[Category: Structural genomics consortium]]
+
-
[[Category: Thiol protease]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Ubiquitin biology]]
+
-
[[Category: Ubiquitin-specific protease activity]]
+
-
[[Category: Ubl conjugation pathway]]
+

Current revision

Usp21 in complex with a ubiquitin-based, USP21-specific inhibitor

PDB ID 3mtn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mtn"
Personal tools