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1haa

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[[Image:1haa.png|left|200px]]
 
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{{STRUCTURE_1haa| PDB=1haa | SCENE= }}
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==A beta-Hairpin Structure in a 13-mer Peptide that Binds a-Bungarotoxin with High Affinity and Neutralizes its Toxicity==
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<StructureSection load='1haa' size='340' side='right'caption='[[1haa]]' scene=''>
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===A BETA-HAIRPIN STRUCTURE IN A 13-MER PEPTIDE THAT BINDS A-BUNGAROTOXIN WITH HIGH AFFINITY AND NEUTRALIZES ITS TOXICITY===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1haa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HAA FirstGlance]. <br>
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{{ABSTRACT_PUBMED_11381118}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1haa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1haa OCA], [https://pdbe.org/1haa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1haa RCSB], [https://www.ebi.ac.uk/pdbsum/1haa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1haa ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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[[1haa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAA OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/1haa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1haa ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[Bungarotoxin|Bungarotoxin]]
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*[[Bungarotoxin 3D structures|Bungarotoxin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011381118</ref><ref group="xtra">PMID:011251289</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bungarus multicinctus]]
[[Category: Bungarus multicinctus]]
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[[Category: Balass, M.]]
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[[Category: Large Structures]]
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[[Category: Fridkin, M.]]
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[[Category: Synthetic construct]]
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[[Category: Fuchs, S.]]
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[[Category: Balass M]]
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[[Category: Kasher, R.]]
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[[Category: Fridkin M]]
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[[Category: Katchalski-Katzir, E.]]
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[[Category: Fuchs S]]
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[[Category: Scherf, T.]]
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[[Category: Kasher R]]
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[[Category: Acetylcholine receptor mimitope]]
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[[Category: Katchalski-Katzir E]]
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[[Category: Alpha-bungarotoxin]]
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[[Category: Scherf T]]
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[[Category: Beta-hairpin]]
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[[Category: Protein-peptide complex]]
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[[Category: Toxin]]
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[[Category: Toxin-peptide complex]]
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[[Category: Toxin/peptide]]
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Current revision

A beta-Hairpin Structure in a 13-mer Peptide that Binds a-Bungarotoxin with High Affinity and Neutralizes its Toxicity

PDB ID 1haa

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