This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1l7j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:30, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1l7j.gif|left|200px]]<br /><applet load="1l7j" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1l7j, resolution 1.90&Aring;" />
 
-
'''X-ray structure of galactose mutarotase from Lactococcus lactis (apo)'''<br />
 
-
==Overview==
+
==X-ray structure of galactose mutarotase from Lactococcus lactis (apo)==
-
Galactose mutarotase plays a key role in normal galactose metabolism by catalyzing the interconversion of beta-D-galactose and alpha-D-galactose. Here we describe the three-dimensional architecture of galactose mutarotase from Lactococcus lactis determined to 1.9-A resolution. Each subunit of the dimeric enzyme displays a distinctive beta-sandwich motif. This tertiary structural element was first identified in beta-galactosidase and subsequently observed in copper amine oxidase, hyaluronate lyase, chondroitinase, and maltose phosphorylase. Two cis-peptides are found in each subunit, namely Pro(67) and Lys(136). The active site is positioned in a rather open cleft, and the electron density corresponding to the bound galactose unequivocally demonstrates that both anomers of the substrate are present in the crystalline enzyme. Those residues responsible for anchoring the sugar to the protein include Arg(71), His(96), His(170), Asp(243), and Glu(304). Both His(96) and His(170) are strictly conserved among mutarotase amino acid sequences determined thus far. The imidazole nitrogens of these residues are located within hydrogen bonding distance to the C-5 oxygen of galactose. Strikingly, the carboxylate group of Glu(304) is situated at approximately 2.7 A from the 1'-hydroxyl group of galactose, thereby suggesting its possible role as a general acid/base group.
+
<StructureSection load='1l7j' size='340' side='right'caption='[[1l7j]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1l7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L7J FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l7j OCA], [https://pdbe.org/1l7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l7j RCSB], [https://www.ebi.ac.uk/pdbsum/1l7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l7j ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q9ZB17_9LACT Q9ZB17_9LACT]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l7/1l7j_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l7j ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1L7J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Active as [http://en.wikipedia.org/wiki/Aldose_1-epimerase Aldose 1-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.3 5.1.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7J OCA].
+
*[[Galactose mutarotase|Galactose mutarotase]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
High resolution X-ray structure of galactose mutarotase from Lactococcus lactis., Thoden JB, Holden HM, J Biol Chem. 2002 Jun 7;277(23):20854-61. Epub 2002 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11907040 11907040]
+
-
[[Category: Aldose 1-epimerase]]
+
[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Holden, H M.]]
+
[[Category: Holden HM]]
-
[[Category: Thoden, J B.]]
+
[[Category: Thoden JB]]
-
[[Category: epimerase]]
+
-
[[Category: galactose metabolism]]
+
-
[[Category: mutarotase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:42:10 2008''
+

Current revision

X-ray structure of galactose mutarotase from Lactococcus lactis (apo)

PDB ID 1l7j

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l7j"
Personal tools