2ix8

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[[Image:2ix8.png|left|200px]]
 
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{{STRUCTURE_2ix8| PDB=2ix8 | SCENE= }}
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==MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME==
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<SX load='2ix8' size='340' side='right' viewer='molstar' caption='[[2ix8]], [[Resolution|resolution]] 9.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ix8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IX8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ix8 OCA], [https://pdbe.org/2ix8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ix8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ix8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ix8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EF3A_YEAST EF3A_YEAST] Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.<ref>PMID:6456269</ref> <ref>PMID:7657623</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/2ix8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ix8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Elongation factor eEF3 is an ATPase that, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aminoacyl-tRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate the clearance of deacyl-tRNA from the E-site. Here we present the crystal structure of Saccharomyces cerevisiae eEF3, showing that it consists of an amino-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains, with a chromodomain inserted in ABC2. Moreover, we present the cryo-electron microscopy structure of the ATP-bound form of eEF3 in complex with the post-translocational-state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release.
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===MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME===
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Structure of eEF3 and the mechanism of transfer RNA release from the E-site.,Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R Nature. 2006 Oct 12;443(7112):663-8. Epub 2006 Aug 23. PMID:16929303<ref>PMID:16929303</ref>
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{{ABSTRACT_PUBMED_16929303}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2ix8" style="background-color:#fffaf0;"></div>
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[[2ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IX8 OCA].
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==See Also==
==See Also==
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*[[Elongation factor|Elongation factor]]
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016929303</ref><references group="xtra"/>
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__TOC__
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</SX>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Anand, M.]]
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[[Category: Anand M]]
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[[Category: Andersen, C B.F.]]
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[[Category: Andersen CBF]]
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[[Category: Andersen, G R.]]
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[[Category: Andersen GR]]
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[[Category: Balar, B.]]
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[[Category: Balar B]]
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[[Category: Becker, T.]]
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[[Category: Becker T]]
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[[Category: Beckmann, R.]]
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[[Category: Beckmann R]]
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[[Category: Blau, M.]]
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[[Category: Blau M]]
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[[Category: Boesen, T.]]
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[[Category: Boesen T]]
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[[Category: Halic, M.]]
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[[Category: Halic M]]
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[[Category: Kinzy, T G.]]
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[[Category: Kinzy TG]]
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[[Category: Mielke, T.]]
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[[Category: Mielke T]]
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[[Category: Pedersen, J S.]]
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[[Category: Pedersen JS]]
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[[Category: Spahn, C M.T.]]
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[[Category: Spahn CMT]]
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[[Category: Atp-binding]]
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[[Category: Elongation factor]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphorylation]]
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[[Category: Protein biosynthesis]]
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[[Category: Rna-binding]]
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[[Category: Rrna-binding]]
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Current revision

MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME

2ix8, resolution 9.90Å

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