2y5y

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[[Image:2y5y.png|left|200px]]
 
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{{STRUCTURE_2y5y| PDB=2y5y | SCENE= }}
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==Crystal structure of LacY in complex with an affinity inactivator==
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<StructureSection load='2y5y' size='340' side='right'caption='[[2y5y]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y5y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y5Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=TGA:METHANETHIOSULFONYL-GALACTOSIDE'>TGA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y5y OCA], [https://pdbe.org/2y5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y5y RCSB], [https://www.ebi.ac.uk/pdbsum/2y5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y5y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lactose permease of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique suicide substrates. In order to study the mechanism of inactivation more precisely, we solved the structure of single-Cys122 LacY in complex with covalently bound MTS-gal. This structure exhibits an inward-facing conformation similar to that observed previously with a slight narrowing of the cytoplasmic cavity. MTS-gal is bound covalently, forming a disulfide bond with C122 and positioned between R144 and W151. E269, a residue essential for binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety. The location of the sugar is in accord with many biochemical studies.
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===CRYSTAL STRUCTURE OF LACY IN COMPLEX WITH AN AFFINITY INACTIVATOR===
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Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.,Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. Epub 2011 May 18. PMID:21593407<ref>PMID:21593407</ref>
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{{ABSTRACT_PUBMED_21593407}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2y5y" style="background-color:#fffaf0;"></div>
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[[2y5y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA].
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==See Also==
==See Also==
*[[Lactose Permease|Lactose Permease]]
*[[Lactose Permease|Lactose Permease]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021593407</ref><ref group="xtra">PMID:012471022</ref><references group="xtra"/>
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__TOC__
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[[Category: Escherichia coli]]
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</StructureSection>
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[[Category: Abramson, J.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Chaptal, V.]]
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[[Category: Large Structures]]
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[[Category: Guan, L.]]
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[[Category: Abramson J]]
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[[Category: Kaback, H R.]]
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[[Category: Chaptal V]]
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[[Category: Kwon, S.]]
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[[Category: Guan L]]
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[[Category: Sawaya, M R.]]
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[[Category: Kaback HR]]
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[[Category: Affinity inactivation]]
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[[Category: Kwon S]]
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[[Category: Transport protein]]
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[[Category: Sawaya MR]]

Current revision

Crystal structure of LacY in complex with an affinity inactivator

PDB ID 2y5y

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