1la6

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[[Image:1la6.gif|left|200px]]<br /><applet load="1la6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1la6, resolution 2.0&Aring;" />
 
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'''The crystal structure of Trematomus newnesi hemoglobin in a partial hemichrome state'''<br />
 
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==Overview==
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==The crystal structure of Trematomus newnesi hemoglobin in a partial hemichrome state==
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<StructureSection load='1la6' size='340' side='right'caption='[[1la6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1la6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LA6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1la6 OCA], [https://pdbe.org/1la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1la6 RCSB], [https://www.ebi.ac.uk/pdbsum/1la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1la6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HBA1_TRENE HBA1_TRENE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/1la6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1la6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Tetrameric hemoglobins are the most widely used systems in studying protein cooperativity. Allosteric effects in hemoglobins arise from the switch between a relaxed (R) state and a tense (T) state occurring upon oxygen release. Here we report the 2.0-A crystal structure of the main hemoglobin component of the Antarctic fish Trematomus newnesi, in a partial hemichrome form. The two alpha-subunit iron atoms are bound to a CO molecule, whereas in the beta subunits the distal histidine residue is the sixth ligand of the heme iron. This structure, a tetrameric hemoglobin in the hemichrome state, demonstrates that the iron coordination by the distal histidine, usually associated with denaturing states, may be tolerated in a native-like hemoglobin structure. In addition, several features of the tertiary and quaternary organization of this structure are intermediate between the R and T states and agree well with the R --&gt; T transition state properties obtained by spectroscopic and kinetic techniques. The analysis of this structure provides a detailed pathway of heme-heme communication and it indicates that the plasticity of the beta heme pocket plays a role in the R --&gt; T transition of tetrameric hemoglobins.
Tetrameric hemoglobins are the most widely used systems in studying protein cooperativity. Allosteric effects in hemoglobins arise from the switch between a relaxed (R) state and a tense (T) state occurring upon oxygen release. Here we report the 2.0-A crystal structure of the main hemoglobin component of the Antarctic fish Trematomus newnesi, in a partial hemichrome form. The two alpha-subunit iron atoms are bound to a CO molecule, whereas in the beta subunits the distal histidine residue is the sixth ligand of the heme iron. This structure, a tetrameric hemoglobin in the hemichrome state, demonstrates that the iron coordination by the distal histidine, usually associated with denaturing states, may be tolerated in a native-like hemoglobin structure. In addition, several features of the tertiary and quaternary organization of this structure are intermediate between the R and T states and agree well with the R --&gt; T transition state properties obtained by spectroscopic and kinetic techniques. The analysis of this structure provides a detailed pathway of heme-heme communication and it indicates that the plasticity of the beta heme pocket plays a role in the R --&gt; T transition of tetrameric hemoglobins.
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==About this Structure==
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The crystal structure of a tetrameric hemoglobin in a partial hemichrome state.,Riccio A, Vitagliano L, di Prisco G, Zagari A, Mazzarella L Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9801-6. Epub 2002 Jul 1. PMID:12093902<ref>PMID:12093902</ref>
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1LA6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi] with <scene name='pdbligand=ACE:'>ACE</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=CMO:'>CMO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LA6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of a tetrameric hemoglobin in a partial hemichrome state., Riccio A, Vitagliano L, di Prisco G, Zagari A, Mazzarella L, Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9801-6. Epub 2002 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12093902 12093902]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1la6" style="background-color:#fffaf0;"></div>
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[[Category: Trematomus newnesi]]
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[[Category: Mazzarella, L.]]
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[[Category: Prisco, G di.]]
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[[Category: Riccio, A.]]
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[[Category: Vitagliano, L.]]
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[[Category: Zagari, A.]]
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[[Category: ACE]]
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[[Category: CMO]]
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[[Category: HEM]]
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[[Category: bishistidine complex]]
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[[Category: hemichrome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:00 2008''
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==See Also==
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Trematomus newnesi]]
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[[Category: Mazzarella L]]
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[[Category: Riccio A]]
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[[Category: Vitagliano L]]
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[[Category: Zagari A]]
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[[Category: Di Prisco G]]

Current revision

The crystal structure of Trematomus newnesi hemoglobin in a partial hemichrome state

PDB ID 1la6

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