2l8k

From Proteopedia

(Difference between revisions)

Current revision

NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)

Structural highlights

2l8k is a 1 chain structure with sequence from Equine arteritis virus Bucyrus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPOA_EAVBU The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.^[1] Nsp1 is essential for viral subgenomic mRNA synthesis.^[2] Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.^[3] The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.^[4] The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.^[5]

Publication Abstract from PubMed

Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7alpha and nsp7beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7alpha for viral RNA synthesis, thus providing a basis for future studies.

Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}.,Manolaridis I, Gaudin C, Posthuma CC, Zevenhoven-Dobbe JC, Imbert I, Canard B, Kelly G, Tucker PA, Conte MR, Snijder EJ J Virol. 2011 Jul;85(14):7449-53. Epub 2011 May 11. PMID:21561912^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Manolaridis I, Gaudin C, Posthuma CC, Zevenhoven-Dobbe JC, Imbert I, Canard B, Kelly G, Tucker PA, Conte MR, Snijder EJ. Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}. J Virol. 2011 Jul;85(14):7449-53. Epub 2011 May 11. PMID:21561912 doi:10.1128/JVI.00255-11

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l8k"

@@ Line 1: / Line 1: @@
-[[Image:2l8k.png|left|200px]]
-{{STRUCTURE_2l8k|  PDB=2l8k  |  SCENE=  }}
+==NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)==
+<StructureSection load='2l8k' size='340' side='right'caption='[[2l8k]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2l8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_arteritis_virus_Bucyrus Equine arteritis virus Bucyrus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8K FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8k OCA], [https://pdbe.org/2l8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8k RCSB], [https://www.ebi.ac.uk/pdbsum/2l8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref>   Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref>   Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref>   The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref>   The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7alpha and nsp7beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7alpha for viral RNA synthesis, thus providing a basis for future studies.
-===NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)===
+Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}.,Manolaridis I, Gaudin C, Posthuma CC, Zevenhoven-Dobbe JC, Imbert I, Canard B, Kelly G, Tucker PA, Conte MR, Snijder EJ J Virol. 2011 Jul;85(14):7449-53. Epub 2011 May 11. PMID:21561912<ref>PMID:21561912</ref>
-{{ABSTRACT_PUBMED_21561912}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2l8k" style="background-color:#fffaf0;"></div>
-[[2l8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Equine_arteritis_virus Equine arteritis virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8K OCA].
 ==See Also==
-*[[Nonstructural protein|Nonstructural protein]]
+*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021561912</ref><references group="xtra"/>
+__TOC__
-[[Category: Equine arteritis virus]]
+</StructureSection>
-[[Category: Conte, M R.]]
+[[Category: Equine arteritis virus Bucyrus]]
-[[Category: Gaudin, C.]]
+[[Category: Large Structures]]
-[[Category: Kelly, G.]]
+[[Category: Conte MR]]
-[[Category: Manolaridis, I.]]
+[[Category: Gaudin C]]
-[[Category: Tucker, P W.]]
+[[Category: Kelly G]]
-[[Category: Viral protein]]
+[[Category: Manolaridis I]]
+[[Category: Tucker PW]]

2l8k

From Proteopedia

Current revision

NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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