1lbi

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[[Image:1lbi.jpg|left|200px]]<br /><applet load="1lbi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lbi, resolution 2.7&Aring;" />
 
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'''LAC REPRESSOR'''<br />
 
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==Overview==
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==LAC REPRESSOR==
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The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.
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<StructureSection load='1lbi' size='340' side='right'caption='[[1lbi]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lbi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LBI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lbi OCA], [https://pdbe.org/1lbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lbi RCSB], [https://www.ebi.ac.uk/pdbsum/1lbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lbi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lb/1lbi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lbi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LBI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LBI OCA].
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*[[Lac repressor|Lac repressor]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the lactose operon repressor and its complexes with DNA and inducer., Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P, Science. 1996 Mar 1;271(5253):1247-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8638105 8638105]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, G.]]
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[[Category: Chang G]]
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[[Category: Horton, N C.]]
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[[Category: Horton NC]]
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[[Category: Kercher, M A.]]
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[[Category: Kercher MA]]
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[[Category: Lewis, M.]]
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[[Category: Lewis M]]
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[[Category: Lu, P.]]
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[[Category: Lu P]]
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[[Category: Pace, H C.]]
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[[Category: Pace HC]]
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[[Category: dna-binding]]
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[[Category: repressor]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:24 2008''
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Current revision

LAC REPRESSOR

PDB ID 1lbi

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