1lc6
From Proteopedia
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| - | [[Image:1lc6.gif|left|200px]]<br /><applet load="1lc6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1lc6" /> | ||
| - | '''Solution Structure of the U6 Intramolecular Stem-loop RNA'''<br /> | ||
| - | == | + | ==Solution Structure of the U6 Intramolecular Stem-loop RNA== |
| + | <StructureSection load='1lc6' size='340' side='right'caption='[[1lc6]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1lc6]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LC6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc6 OCA], [https://pdbe.org/1lc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1lc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lc6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing. | U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing. | ||
| - | + | Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure.,Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:11992125<ref>PMID:11992125</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1lc6" style="background-color:#fffaf0;"></div> |
| - | [[Category: Allmann | + | == References == |
| - | [[Category: Brow | + | <references/> |
| - | [[Category: Butcher | + | __TOC__ |
| - | [[Category: Huppler | + | </StructureSection> |
| - | [[Category: Nikstad | + | [[Category: Large Structures]] |
| - | + | [[Category: Allmann AM]] | |
| - | + | [[Category: Brow DA]] | |
| - | + | [[Category: Butcher SE]] | |
| - | + | [[Category: Huppler A]] | |
| - | + | [[Category: Nikstad LJ]] | |
Current revision
Solution Structure of the U6 Intramolecular Stem-loop RNA
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