2p2c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:25, 17 October 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2p2c.png|left|200px]]
 
-
{{STRUCTURE_2p2c| PDB=2p2c | SCENE= }}
+
==Inhibition of caspase-2 by a designed ankyrin repeat protein (DARPin)==
 +
<StructureSection load='2p2c' size='340' side='right'caption='[[2p2c]], [[Resolution|resolution]] 3.24&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2p2c]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P2C FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.24&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p2c OCA], [https://pdbe.org/2p2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p2c RCSB], [https://www.ebi.ac.uk/pdbsum/2p2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p2c ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CASP2_HUMAN CASP2_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p2/2p2c_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p2c ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Specific and potent caspase inhibitors are indispensable for the dissection of the intricate pathways leading to apoptosis. We selected a designed ankyrin repeat protein (DARPin) from a combinatorial library that inhibits caspase-2 in vitro with a subnanomolar inhibition constant and, in contrast to the peptidic caspase inhibitors, with very high specificity for this particular caspase. The crystal structure of this inhibitor (AR_F8) in complex with caspase-2 reveals the molecular basis for the specificity and, together with kinetic analyses, the allosteric mechanism of inhibition. The structure also shows a conformation of the active site that can be exploited for the design of inhibitory compounds. AR_F8 is a specific inhibitor of an initiator caspase and has the potential to help identify the function of caspase-2 in the complex biological apoptotic signaling network.
-
===Inhibition of caspase-2 by a designed ankyrin repeat protein (DARPin)===
+
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism.,Schweizer A, Roschitzki-Voser H, Amstutz P, Briand C, Gulotti-Georgieva M, Prenosil E, Binz HK, Capitani G, Baici A, Pluckthun A, Grutter MG Structure. 2007 May;15(5):625-36. PMID:17502107<ref>PMID:17502107</ref>
-
{{ABSTRACT_PUBMED_17502107}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2p2c" style="background-color:#fffaf0;"></div>
-
[[2p2c]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2C OCA].
+
==See Also==
==See Also==
-
*[[Caspase|Caspase]]
+
*[[Caspase 3D structures|Caspase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:017502107</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Briand, C.]]
+
[[Category: Large Structures]]
-
[[Category: Capitani, G.]]
+
[[Category: Briand C]]
-
[[Category: Gruetter, M G.]]
+
[[Category: Capitani G]]
-
[[Category: Voser, HRoschitzki.]]
+
[[Category: Gruetter MG]]
-
[[Category: Apoptosis]]
+
[[Category: Roschitzki Voser H]]
-
[[Category: Caspase]]
+
-
[[Category: Caspase-2]]
+
-
[[Category: Designed ankyrin repeat protein]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Inhibition]]
+
-
[[Category: Protein design]]
+
-
[[Category: Protein library]]
+
-
[[Category: Ribosome display]]
+

Current revision

Inhibition of caspase-2 by a designed ankyrin repeat protein (DARPin)

PDB ID 2p2c

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p2c"
Personal tools