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1leh

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[[Image:1leh.gif|left|200px]]<br /><applet load="1leh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1leh, resolution 2.2&Aring;" />
 
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'''LEUCINE DEHYDROGENASE FROM BACILLUS SPHAERICUS'''<br />
 
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==Overview==
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==LEUCINE DEHYDROGENASE FROM BACILLUS SPHAERICUS==
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BACKGROUND: Glutamate, phenylalanine and leucine dehydrogenases catalyze the NAD(P)(+)-linked oxidative deamination of L-amino acids to the corresponding 2-oxoacids, and sequence homology between these enzymes clearly indicates the existence of an enzyme superfamily related by divergent evolution. We have undertaken structural studies on a number of members of this family in order to investigate the molecular basis of their differential amino acid specificity. RESULTS: We have solved the X-ray structure of the leucine dehydrogenase from Bacillus sphaericus to a resolution of 2.2 A. Each subunit of this octameric enzyme contains 364 amino acids and folds into two domains, separated by a deep cleft. The nicotinamide ring of the NAD+ cofactor binds deep in this cleft, which is thought to close during the hydride transfer step of the catalytic cycle. CONCLUSIONS: Comparison of the structure of leucine dehydrogenase with a hexameric glutamate dehydrogenase has shown that these two enzymes share a related fold and possess a similar catalytic chemistry. A mechanism for the basis of the differential amino acid specificity between these enzymes involves point mutations in the amino acid side-chain specificity pocket and subtle changes in the shape of this pocket caused by the differences in quaternary structure.
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<StructureSection load='1leh' size='340' side='right'caption='[[1leh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1leh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LEH FirstGlance]. <br>
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1LEH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Active as [http://en.wikipedia.org/wiki/Leucine_dehydrogenase Leucine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.9 1.4.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1leh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1leh OCA], [https://pdbe.org/1leh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1leh RCSB], [https://www.ebi.ac.uk/pdbsum/1leh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1leh ProSAT]</span></td></tr>
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==Reference==
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</table>
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A role for quaternary structure in the substrate specificity of leucine dehydrogenase., Baker PJ, Turnbull AP, Sedelnikova SE, Stillman TJ, Rice DW, Structure. 1995 Jul 15;3(7):693-705. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8591046 8591046]
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== Function ==
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[[Category: Leucine dehydrogenase]]
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[https://www.uniprot.org/uniprot/Q7SIB4_LYSSH Q7SIB4_LYSSH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1leh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1leh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Lysinibacillus sphaericus]]
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[[Category: Single protein]]
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[[Category: Baker PJ]]
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[[Category: Baker, P J.]]
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[[Category: Rice DW]]
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[[Category: Rice, D W.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S E.]]
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[[Category: Stillman TJ]]
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[[Category: Stillman, T J.]]
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[[Category: Turnbull AP]]
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[[Category: Turnbull, A P.]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:18 2008''
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Current revision

LEUCINE DEHYDROGENASE FROM BACILLUS SPHAERICUS

PDB ID 1leh

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