3hde

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[[Image:3hde.png|left|200px]]
 
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{{STRUCTURE_3hde| PDB=3hde | SCENE= }}
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==Crystal structure of full-length endolysin R21 from phage 21==
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<StructureSection load='3hde' size='340' side='right'caption='[[3hde]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hde]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_P21 Enterobacteria phage P21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HDE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hde OCA], [https://pdbe.org/3hde PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hde RCSB], [https://www.ebi.ac.uk/pdbsum/3hde PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hde ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ENLYS_BPP21 ENLYS_BPP21] Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the pinholin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. Once the pinholin has permeabilized the host cell membrane, the SAR-endolysin is released into the periplasm where it breaks down the peptidoglycan layer.[HAMAP-Rule:MF_04136]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/3hde_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hde ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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R(21), the lysozyme of coliphage 21, has an N-terminal signal-anchor-release (SAR) domain that directs its secretion in a membrane-tethered, inactive form and then its release and activation in the periplasm. Both genetic and crystallographic studies show that the SAR domain, once extracted from the bilayer, refolds into the body of the enzyme and effects muralytic activation by repositioning one residue of the canonical lysozyme catalytic triad.
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===Crystal structure of full-length endolysin R21 from phage 21===
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Regulation of a muralytic enzyme by dynamic membrane topology.,Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC Nat Struct Mol Biol. 2009 Nov;16(11):1192-4. Epub 2009 Nov 1. PMID:19881499<ref>PMID:19881499</ref>
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{{ABSTRACT_PUBMED_19881499}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3hde" style="background-color:#fffaf0;"></div>
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[[3hde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDE OCA].
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==See Also==
==See Also==
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*[[Hen Egg-White (HEW) Lysozyme|Hen Egg-White (HEW) Lysozyme]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019881499</ref><references group="xtra"/>
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__TOC__
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[[Category: Enterobacteria phage p21]]
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</StructureSection>
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[[Category: Lysozyme]]
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[[Category: Enterobacteria phage P21]]
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[[Category: Arockiasamy, A.]]
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[[Category: Large Structures]]
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[[Category: Caronna, E.]]
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[[Category: Arockiasamy A]]
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[[Category: McKee, E.]]
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[[Category: Caronna E]]
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[[Category: Sacchettini, J C.]]
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[[Category: McKee E]]
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[[Category: Sun, Q.]]
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[[Category: Sacchettini JC]]
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[[Category: Antimicrobial]]
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[[Category: Sun Q]]
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[[Category: Bacteriolytic enzyme]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Late protein]]
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[[Category: Lysozyme-like]]
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Current revision

Crystal structure of full-length endolysin R21 from phage 21

PDB ID 3hde

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