3jtd

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[[Image:3jtd.png|left|200px]]
 
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{{STRUCTURE_3jtd| PDB=3jtd | SCENE= }}
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==Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation==
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<StructureSection load='3jtd' size='340' side='right'caption='[[3jtd]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jtd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JTD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jtd OCA], [https://pdbe.org/3jtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jtd RCSB], [https://www.ebi.ac.uk/pdbsum/3jtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jtd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYS_ARGIR MYS_ARGIR] Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jtd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In regulated myosin, motor and enzymatic activities are toggled between the on-state and off-state by a switch located on its lever arm domain, here called the regulatory domain (RD). This region consists of a long alpha-helical "heavy chain" stabilized by a "regulatory" light chain (RLC) and an "essential" light chain (ELC). The on-state is activated by phosphorylation of the RLC of vertebrate smooth muscle RD or by direct binding of Ca(2+) to the ELC of molluscan RD. Crystal structures are available only for the molluscan RD. To understand in more detail the pathway between the on-state and the off-state, we have now also determined the crystal structure of a molluscan (scallop) RD in the absence of Ca(2+). Our results indicate that loss of Ca(2+) abolishes most of the interactions between the light chains and may increase the flexibility of the RD heavy chain. We propose that disruption of critical links with the C-lobe of the RLC is the key event initiating the off-state in both smooth muscle myosins and molluscan myosins.
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===Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation===
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The on-off switch in regulated myosins: different triggers but related mechanisms.,Himmel DM, Mui S, O'Neall-Hennessey E, Szent-Gyorgyi AG, Cohen C J Mol Biol. 2009 Dec 4;394(3):496-505. Epub 2009 Sep 19. PMID:19769984<ref>PMID:19769984</ref>
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{{ABSTRACT_PUBMED_19769984}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3jtd" style="background-color:#fffaf0;"></div>
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[[3jtd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTD OCA].
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==See Also==
==See Also==
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*[[Myosin|Myosin]]
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019769984</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Argopecten irradians]]
[[Category: Argopecten irradians]]
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[[Category: Cohen, C.]]
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[[Category: Large Structures]]
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[[Category: Himmel, D M.]]
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[[Category: Cohen C]]
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[[Category: Mui, S.]]
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[[Category: Himmel DM]]
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[[Category: Neall-Hennessey, E O.]]
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[[Category: Mui S]]
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[[Category: Szent-Gyorgyi, A.]]
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[[Category: O'Neall-Hennessey E]]
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[[Category: Actin-binding]]
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[[Category: Szent-Gyorgyi A]]
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[[Category: Atp-binding]]
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[[Category: Calmodulin-binding]]
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[[Category: Contractile protein]]
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[[Category: Motor protein]]
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[[Category: Muscle protein]]
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[[Category: Myosin]]
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[[Category: Nucleotide-binding]]
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[[Category: Off-state]]
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[[Category: Regulated myosin]]
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[[Category: Scallop regulatory domain/lever arm]]
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[[Category: Smooth and molluscan muscle]]
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[[Category: Thick filament]]
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[[Category: X-ray crystallographic structure]]
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Current revision

Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation

PDB ID 3jtd

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