3m0b

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[[Image:3m0b.png|left|200px]]
 
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{{STRUCTURE_3m0b| PDB=3m0b | SCENE= }}
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==Ru-Porphyrin Protein Scaffolds for Sensing O2==
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<StructureSection load='3m0b' size='340' side='right'caption='[[3m0b]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3m0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M0B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=RUR:[3,3-(7,12-DIETHYL-3,8,13,17-TETRAMETHYLPORPHYRIN-2,18-DIYL-KAPPA~4~N~21~,N~22~,N~23~,N~24~)DIPROPANOATO(2-)]RUTHENIUM'>RUR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0b OCA], [https://pdbe.org/3m0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m0b RCSB], [https://www.ebi.ac.uk/pdbsum/3m0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m0/3m0b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m0b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hemoprotein-based scaffolds containing phosphorescent ruthenium(II) CO mesoporphyrin IX (RuMP) are reported here for oxygen (O(2)) sensing in biological contexts. RuMP was incorporated into the protein scaffolds during protein expression utilizing a novel method that we have described previously. A high-resolution (2.00 A) crystal structure revealed that the unnatural porphyrin binds to the proteins in a manner similar to the native heme and does not perturb the protein fold. The protein scaffolds were found to provide unique coordination environments for RuMP and modulate the porphyrin emission properties. Emission lifetime measurements demonstrate a linear O(2) response within the physiological range and precision comparable to commercial O(2) sensors. The RuMP proteins are robust, readily modifiable platforms and display promising O(2) sensing properties for future in vivo applications.
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===Ru-Porphyrin Protein Scaffolds for Sensing O2===
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Ru-porphyrin protein scaffolds for sensing O2.,Winter MB, McLaurin EJ, Reece SY, Olea C Jr, Nocera DG, Marletta MA J Am Chem Soc. 2010 Apr 28;132(16):5582-3. PMID:20373741<ref>PMID:20373741</ref>
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{{ABSTRACT_PUBMED_20373741}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3m0b" style="background-color:#fffaf0;"></div>
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[[3m0b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0B OCA].
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==See Also==
==See Also==
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*[[Chemotaxis protein|Chemotaxis protein]]
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*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
*[[Methyl-accepting chemotaxis protein|Methyl-accepting chemotaxis protein]]
*[[Methyl-accepting chemotaxis protein|Methyl-accepting chemotaxis protein]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020373741</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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[[Category: Marletta, M A.]]
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[[Category: Large Structures]]
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[[Category: McLaurin, E J.]]
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[[Category: Marletta MA]]
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[[Category: Nocera, D G.]]
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[[Category: McLaurin EJ]]
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[[Category: Olea, C.]]
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[[Category: Nocera DG]]
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[[Category: Reece, S Y.]]
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[[Category: Olea Jr C]]
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[[Category: Winter, M B.]]
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[[Category: Reece SY]]
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[[Category: Oxygen sensing molecule]]
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[[Category: Winter MB]]
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[[Category: Signaling protein]]
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Current revision

Ru-Porphyrin Protein Scaffolds for Sensing O2

PDB ID 3m0b

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