2zfk

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[[Image:2zfk.png|left|200px]]
 
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{{STRUCTURE_2zfk| PDB=2zfk | SCENE= }}
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==Crystal Structure of the Kif1A Motor Domain during Mg release: Mg-releasing Transition-2==
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<StructureSection load='2zfk' size='340' side='right'caption='[[2zfk]], [[Resolution|resolution]] 3.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2zfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZFK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfk OCA], [https://pdbe.org/2zfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zfk RCSB], [https://www.ebi.ac.uk/pdbsum/2zfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KIF1A_MOUSE KIF1A_MOUSE] Motor for anterograde axonal transport of synaptic vesicle precursors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zfk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zfk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mg-ADP release is considered to be a crucial process for the regulation and motility of kinesin. To gain insight into the structural basis of this process, we solved the atomic structures of kinesin superfamily protein-1A (KIF1A) during and after Mg(2+) release. On the basis of new structural and mutagenesis data, we propose a model mechanism for microtubule activation of Mg-ADP release from KIF1A. In our model, a specific interaction between loop L7 of KIF1A and beta-tubulin reconfigures the KIF1A active site by shifting the relative positions of switches I and II. This leads to the sequential release of a group of water molecules that sits over the Mg(2+) in the active site, followed by Mg(2+) and finally the ADP. We further propose that this set of events is linked to a strain-dependent docking of the neck linker to the motor core, which produces a two-step power stroke.
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===Crystal Structure of the Kif1A Motor Domain during Mg release: Mg-releasing Transition-2===
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Structural model for strain-dependent microtubule activation of Mg-ADP release from kinesin.,Nitta R, Okada Y, Hirokawa N Nat Struct Mol Biol. 2008 Oct;15(10):1067-75. Epub 2008 Sep 21. PMID:18806800<ref>PMID:18806800</ref>
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{{ABSTRACT_PUBMED_18806800}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2zfk" style="background-color:#fffaf0;"></div>
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[[2zfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFK OCA].
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==See Also==
==See Also==
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*[[Kinesin|Kinesin]]
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018806800</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Hirokawa, N.]]
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[[Category: Hirokawa N]]
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[[Category: Nitta, R.]]
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[[Category: Nitta R]]
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[[Category: Okada, Y.]]
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[[Category: Okada Y]]
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[[Category: Alpha and beta protein]]
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[[Category: Atp-binding]]
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[[Category: Atpase]]
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[[Category: Enzyme]]
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[[Category: Kinesin]]
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[[Category: Microtubule]]
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[[Category: Motor protein]]
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[[Category: Nucleotide-binding]]
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[[Category: P-loop]]
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[[Category: Transport protein]]
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Current revision

Crystal Structure of the Kif1A Motor Domain during Mg release: Mg-releasing Transition-2

PDB ID 2zfk

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