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1lrz

From Proteopedia

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[[Image:1lrz.jpg|left|200px]]<br /><applet load="1lrz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lrz, resolution 2.10&Aring;" />
 
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'''x-ray crystal structure of staphylococcus aureus femA'''<br />
 
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==Overview==
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==x-ray crystal structure of staphylococcus aureus femA==
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The latter stages of peptidoglycan biosynthesis in Staphylococci involve the synthesis of a pentaglycine bridge on the epsilon amino group of the pentapeptide lysine side chain. Genetic and biochemical evidence suggest that sequential addition of these glycines is catalyzed by three homologous enzymes, FemX (FmhB), FemA, and FemB. The first protein structure from this family, Staphylococcus aureus FemA, has been solved at 2.1 A resolution by X-ray crystallography. The FemA structure reveals a unique organization of several known protein folds involved in peptide and tRNA binding. The surface of the protein also reveals an L-shaped channel suitable for a peptidoglycan substrate. Analysis of the structural features of this enzyme provides clues to the mechanism of action of S. aureus FemA.
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<StructureSection load='1lrz' size='340' side='right'caption='[[1lrz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lrz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LRZ FirstGlance]. <br>
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1LRZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lrz OCA], [https://pdbe.org/1lrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lrz RCSB], [https://www.ebi.ac.uk/pdbsum/1lrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lrz ProSAT]</span></td></tr>
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==Reference==
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</table>
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X-ray crystal structure of Staphylococcus aureus FemA., Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL, Structure. 2002 Aug;10(8):1107-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12176388 12176388]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/FEMA_STAAU FEMA_STAAU] Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 2 and 3 of the pentaglycine bridge, using glycyl-tRNA(Gly) as donor.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lr/1lrz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lrz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Benson, T.]]
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[[Category: Benson T]]
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[[Category: Choi, G.]]
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[[Category: Choi G]]
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[[Category: Curry, K.]]
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[[Category: Curry K]]
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[[Category: Garlick, R.]]
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[[Category: Garlick R]]
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[[Category: Hagadorn, J.]]
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[[Category: Hagadorn J]]
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[[Category: Ho, A.]]
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[[Category: Ho A]]
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[[Category: Mutchler, V.]]
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[[Category: Mutchler V]]
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[[Category: Prince, D.]]
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[[Category: Prince D]]
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[[Category: Sarver, R.]]
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[[Category: Sarver R]]
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[[Category: multiple anomalous dispersion]]
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[[Category: peptidoglycan]]
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[[Category: staphylococcus aureus]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:01 2008''
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Current revision

x-ray crystal structure of staphylococcus aureus femA

PDB ID 1lrz

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