3a9b

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[[Image:3a9b.png|left|200px]]
 
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{{STRUCTURE_3a9b| PDB=3a9b | SCENE= }}
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==CcCel6C, a glycoside hydrolase family 6 enzyme, complexed with cellobiose==
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<StructureSection load='3a9b' size='340' side='right'caption='[[3a9b]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3a9b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A9B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a9b OCA], [https://pdbe.org/3a9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a9b RCSB], [https://www.ebi.ac.uk/pdbsum/3a9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a9b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B7X9Z2_COPCI B7X9Z2_COPCI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/3a9b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a9b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The basidiomycete Coprinopsis cinerea produces the glycoside hydrolase family 6 enzyme CcCel6C at low and constitutive levels. CcCel6C exhibits unusual cellobiohydrolase activity; it hydrolyses carboxymethyl cellulose, which is a poor substrate for typical cellobiohydrolases. Here, we determined the crystal structures of CcCel6C unbound and in complex with p-nitrophenyl beta-D-cellotrioside and cellobiose. CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, which is observed in other cellobiohydrolases from ascomecetes Hypocrea jecorina (HjeCel6A) and Humicola insolens (HinCel6A). In HjeCel6A and HinCel6A, ligand binding produces a conformational change that narrows this tunnel. In contrast, the tunnel remains wide in CcCel6C and the conformational change appears to be less favourable than in HjeCel6A and HinCel6A. The ligand binding cleft for subsite -3 of CcCel6C is also wide and is rather similar to that of endoglucanase. These results suggest that the open tunnel and the wide cleft are suitable for the hydrolysis of carboxymethyl cellulose.
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===CcCel6C, a glycoside hydrolase family 6 enzyme, complexed with cellobiose===
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Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea.,Liu Y, Yoshida M, Kurakata Y, Miyazaki T, Igarashi K, Samejima M, Fukuda K, Nishikawa A, Tonozuka T FEBS J. 2010 Mar;277(6):1532-42. Epub 2010 Feb 10. PMID:20148970<ref>PMID:20148970</ref>
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{{ABSTRACT_PUBMED_20148970}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3a9b" style="background-color:#fffaf0;"></div>
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[[3a9b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A9B OCA].
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==See Also==
==See Also==
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*[[Cellobiohydrolase|Cellobiohydrolase]]
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*[[Cellobiohydrolase 3D structures|Cellobiohydrolase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020148970</ref><ref group="xtra">PMID:019194005</ref><references group="xtra"/>
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__TOC__
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[[Category: Cellulose 1,4-beta-cellobiosidase]]
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</StructureSection>
[[Category: Coprinopsis cinerea]]
[[Category: Coprinopsis cinerea]]
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[[Category: Kurakata, Y.]]
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[[Category: Large Structures]]
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[[Category: Liu, Y.]]
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[[Category: Kurakata Y]]
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[[Category: Miyazaki, T.]]
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[[Category: Liu Y]]
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[[Category: Nishikawa, A.]]
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[[Category: Miyazaki T]]
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[[Category: Tonozuka, T.]]
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[[Category: Nishikawa A]]
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[[Category: Yoshida, M.]]
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[[Category: Tonozuka T]]
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[[Category: Cellobiohydrolase]]
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[[Category: Yoshida M]]
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[[Category: Cellulase]]
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[[Category: Glycoside hydrolase family 6]]
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[[Category: Hydrolase]]
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[[Category: Seven-stranded beta-alpha barrel]]
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Current revision

CcCel6C, a glycoside hydrolase family 6 enzyme, complexed with cellobiose

PDB ID 3a9b

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