4eiy

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[[Image:4eiy.png|left|200px]]
 
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{{STRUCTURE_4eiy| PDB=4eiy | SCENE= }}
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==Crystal structure of the chimeric protein of A2aAR-BRIL in complex with ZM241385 at 1.8A resolution==
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<StructureSection load='4eiy' size='340' side='right'caption='[[4eiy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4eiy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiy OCA], [https://pdbe.org/4eiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiy RCSB], [https://www.ebi.ac.uk/pdbsum/4eiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pharmacological responses of G protein-coupled receptors (GPCRs) can be fine-tuned by allosteric modulators. Structural studies of such effects have been limited due to the medium resolution of GPCR structures. We reengineered the human A(2A) adenosine receptor by replacing its third intracellular loop with apocytochrome b(562)RIL and solved the structure at 1.8 angstrom resolution. The high-resolution structure allowed us to identify 57 ordered water molecules inside the receptor comprising three major clusters. The central cluster harbors a putative sodium ion bound to the highly conserved aspartate residue Asp(2.50). Additionally, two cholesterols stabilize the conformation of helix VI, and one of 23 ordered lipids intercalates inside the ligand-binding pocket. These high-resolution details shed light on the potential role of structured water molecules, sodium ions, and lipids/cholesterol in GPCR stabilization and function.
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===Crystal structure of the chimeric protein of A2aAR-BRIL in complex with ZM241385 at 1.8A resolution===
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Structural basis for allosteric regulation of GPCRs by sodium ions.,Liu W, Chun E, Thompson AA, Chubukov P, Xu F, Katritch V, Han GW, Roth CB, Heitman LH, IJzerman AP, Cherezov V, Stevens RC Science. 2012 Jul 13;337(6091):232-6. PMID:22798613<ref>PMID:22798613</ref>
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{{ABSTRACT_PUBMED_22798613}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 4eiy" style="background-color:#fffaf0;"></div>
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[[4eiy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIY OCA].
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==See Also==
==See Also==
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*[[G protein-coupled receptor|G protein-coupled receptor]]
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*[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022798613</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Cherezov, V.]]
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[[Category: Large Structures]]
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[[Category: Chubukov, P.]]
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[[Category: Cherezov V]]
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[[Category: Chun, E.]]
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[[Category: Chubukov P]]
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[[Category: GPCR, GPCR Network.]]
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[[Category: Chun E]]
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[[Category: Han, G W.]]
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[[Category: Han GW]]
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[[Category: Heitman, L H.]]
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[[Category: Heitman LH]]
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[[Category: Ijzerman, A P.]]
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[[Category: Ijzerman AP]]
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[[Category: Katritch, V.]]
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[[Category: Katritch V]]
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[[Category: Liu, W.]]
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[[Category: Liu W]]
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[[Category: Stevens, R C.]]
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[[Category: Stevens RC]]
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[[Category: Thompson, A A.]]
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[[Category: Thompson AA]]
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[[Category: Xu, F.]]
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[[Category: Xu F]]
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[[Category: Gpcr]]
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[[Category: Gpcr network]]
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[[Category: Membrane protein]]
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[[Category: Novel protein engineering]]
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[[Category: Psi-biology]]
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[[Category: Structural genomic]]
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Current revision

Crystal structure of the chimeric protein of A2aAR-BRIL in complex with ZM241385 at 1.8A resolution

PDB ID 4eiy

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