1lua

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[[Image:1lua.jpg|left|200px]]<br /><applet load="1lua" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lua, resolution 1.90&Aring;" />
 
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'''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP'''<br />
 
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==Overview==
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==Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP==
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NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.
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<StructureSection load='1lua' size='340' side='right'caption='[[1lua]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lua]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens_AM1 Methylorubrum extorquens AM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LUA FirstGlance]. <br>
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1LUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_dehydrogenase_(NADP(+)) Methylenetetrahydrofolate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.5 1.5.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lua OCA], [https://pdbe.org/1lua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lua RCSB], [https://www.ebi.ac.uk/pdbsum/1lua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lua ProSAT]</span></td></tr>
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Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12176390 12176390]
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</table>
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[[Category: Methylenetetrahydrofolate dehydrogenase (NADP(+))]]
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== Function ==
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[[Category: Methylobacterium extorquens]]
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[https://www.uniprot.org/uniprot/MTDA_METEA MTDA_METEA] Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Demmer, U.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ermler, U.]]
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Check<jmol>
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[[Category: Grabarse, W.]]
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<jmolCheckbox>
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[[Category: Hagemeier, C H.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lu/1lua_consurf.spt"</scriptWhenChecked>
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[[Category: Roth, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Vorholt, J A.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Warkentin, E.]]
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</jmolCheckbox>
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[[Category: NAP]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lua ConSurf].
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[[Category: alpha/beta twisted open sheet structure]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:31 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Methylorubrum extorquens AM1]]
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[[Category: Demmer U]]
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[[Category: Ermler U]]
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[[Category: Grabarse W]]
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[[Category: Hagemeier CH]]
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[[Category: Roth A]]
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[[Category: Vorholt JA]]
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[[Category: Warkentin E]]

Current revision

Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP

PDB ID 1lua

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