3cap

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:40, 21 November 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3cap.png|left|200px]]
 
-
{{STRUCTURE_3cap| PDB=3cap | SCENE= }}
+
==Crystal Structure of Native Opsin: the G Protein-Coupled Receptor Rhodopsin in its Ligand-free State==
 +
<StructureSection load='3cap' size='340' side='right'caption='[[3cap]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3cap]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CAP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGL:B-2-OCTYLGLUCOSIDE'>BGL</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cap OCA], [https://pdbe.org/3cap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cap RCSB], [https://www.ebi.ac.uk/pdbsum/3cap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cap ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/OPSD_BOVIN OPSD_BOVIN] Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).<ref>PMID:16908857</ref> <ref>PMID:17060607</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/3cap_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cap ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
In the G-protein-coupled receptor (GPCR) rhodopsin, the inactivating ligand 11-cis-retinal is bound in the seven-transmembrane helix (TM) bundle and is cis/trans isomerized by light to form active metarhodopsin II. With metarhodopsin II decay, all-trans-retinal is released, and opsin is reloaded with new 11-cis-retinal. Here we present the crystal structure of ligand-free native opsin from bovine retinal rod cells at 2.9 angstrom (A) resolution. Compared to rhodopsin, opsin shows prominent structural changes in the conserved E(D)RY and NPxxY(x)(5,6)F regions and in TM5-TM7. At the cytoplasmic side, TM6 is tilted outwards by 6-7 A, whereas the helix structure of TM5 is more elongated and close to TM6. These structural changes, some of which were attributed to an active GPCR state, reorganize the empty retinal-binding pocket to disclose two openings that may serve the entry and exit of retinal. The opsin structure sheds new light on ligand binding to GPCRs and on GPCR activation.
-
===Crystal Structure of Native Opsin: the G Protein-Coupled Receptor Rhodopsin in its Ligand-free State===
+
Crystal structure of the ligand-free G-protein-coupled receptor opsin.,Park JH, Scheerer P, Hofmann KP, Choe HW, Ernst OP Nature. 2008 Jul 10;454(7201):183-7. Epub 2008 Jun 18. PMID:18563085<ref>PMID:18563085</ref>
-
{{ABSTRACT_PUBMED_18563085}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 3cap" style="background-color:#fffaf0;"></div>
-
[[3cap]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CAP OCA].
+
==See Also==
==See Also==
*[[Rhodopsin|Rhodopsin]]
*[[Rhodopsin|Rhodopsin]]
-
 
+
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
-
==Reference==
+
== References ==
-
<ref group="xtra">PMID:018563085</ref><references group="xtra"/>
+
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Choe, H W.]]
+
[[Category: Large Structures]]
-
[[Category: Ernst, O P.]]
+
[[Category: Choe H-W]]
-
[[Category: Hofmann, K P.]]
+
[[Category: Ernst OP]]
-
[[Category: Park, J H.]]
+
[[Category: Hofmann KP]]
-
[[Category: Scheerer, P.]]
+
[[Category: Park JH]]
-
[[Category: Chromophore]]
+
[[Category: Scheerer P]]
-
[[Category: G protein-coupled receptor]]
+
-
[[Category: G-protein coupled receptor]]
+
-
[[Category: Glycoprotein]]
+
-
[[Category: Ligand-free state]]
+
-
[[Category: Lipoprotein]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: Opsin]]
+
-
[[Category: Palmitate]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Photoreceptor]]
+
-
[[Category: Photoreceptor protein]]
+
-
[[Category: Retinal protein]]
+
-
[[Category: Rhodopsin]]
+
-
[[Category: Sensory transduction]]
+
-
[[Category: Signaling protein]]
+
-
[[Category: Transducer]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Vision]]
+

Current revision

Crystal Structure of Native Opsin: the G Protein-Coupled Receptor Rhodopsin in its Ligand-free State

PDB ID 3cap

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cap"
Personal tools