1kxr

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[[Image:1kxr.png|left|200px]]
 
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{{STRUCTURE_1kxr| PDB=1kxr | SCENE= }}
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==Crystal Structure of Calcium-Bound Protease Core of Calpain I==
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<StructureSection load='1kxr' size='340' side='right'caption='[[1kxr]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kxr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KXR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kxr OCA], [https://pdbe.org/1kxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kxr RCSB], [https://www.ebi.ac.uk/pdbsum/1kxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kxr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAN1_RAT CAN1_RAT] Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/1kxr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kxr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ca(2+) signaling by calpains leads to controlled proteolysis during processes ranging from cytoskeleton remodeling in mammals to sex determination in nematodes. Deregulated Ca(2+) levels result in aberrant proteolysis by calpains, which contributes to tissue damage in heart and brain ischemias as well as neurodegeneration in Alzheimer's disease. Here we show that activation of the protease core of mu calpain requires cooperative binding of two Ca(2+) atoms at two non-EF-hand sites revealed in the 2.1 A crystal structure. Conservation of the Ca(2+) binding residues defines an ancestral general mechanism of activation for most calpain isoforms, including some that lack EF-hand domains. The protease region is not affected by the endogenous inhibitor, calpastatin, and may contribute to calpain-mediated pathologies when the core is released by autoproteolysis.
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===Crystal Structure of Calcium-Bound Protease Core of Calpain I===
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A Ca(2+) switch aligns the active site of calpain.,Moldoveanu T, Hosfield CM, Lim D, Elce JS, Jia Z, Davies PL Cell. 2002 Mar 8;108(5):649-60. PMID:11893336<ref>PMID:11893336</ref>
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{{ABSTRACT_PUBMED_11893336}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1kxr" style="background-color:#fffaf0;"></div>
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[[1kxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXR OCA].
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==See Also==
==See Also==
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*[[Calpain|Calpain]]
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*[[Calpain 3D structures|Calpain 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011893336</ref><ref group="xtra">PMID:012665854</ref><references group="xtra"/>
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__TOC__
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[[Category: Hydrolase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Davies, P L.]]
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[[Category: Davies PL]]
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[[Category: Elce, J S.]]
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[[Category: Elce JS]]
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[[Category: Hosfield, C M.]]
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[[Category: Hosfield CM]]
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[[Category: Jia, Z.]]
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[[Category: Jia Z]]
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[[Category: Lim, D.]]
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[[Category: Lim D]]
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[[Category: Moldoveanu, T.]]
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[[Category: Moldoveanu T]]
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[[Category: Calcium-dependent cysteine protease]]
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[[Category: Hydrolase]]
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[[Category: Papain-related]]
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[[Category: Two novel cooperative calcium site]]
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Current revision

Crystal Structure of Calcium-Bound Protease Core of Calpain I

PDB ID 1kxr

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