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1lx7

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[[Image:1lx7.gif|left|200px]]<br /><applet load="1lx7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lx7, resolution 2.00&Aring;" />
 
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'''Structure of E. coli uridine phosphorylase at 2.0A'''<br />
 
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==Overview==
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==Structure of E. coli uridine phosphorylase at 2.0A==
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The 2.0 A crystal structure has been determined for Escherichia coli uridine phosphorylase (UP), an essential enzyme in nucleotide biosynthesis that catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose-1-phosphate and uracil. The structure determination of two independent monomers in the asymmetric unit revealed the residue composition and atomic details of the apo configurations of each active site. The native hexameric UP enzyme was revealed by applying threefold crystallographic symmetry to the contents of the asymmetric unit. The 2.0 A model reveals a closer structural relationship to other nucleotide phosphorylase enzymes than was previously appreciated.
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<StructureSection load='1lx7' size='340' side='right'caption='[[1lx7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lx7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LX7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lx7 OCA], [https://pdbe.org/1lx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lx7 RCSB], [https://www.ebi.ac.uk/pdbsum/1lx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lx7 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1lx7 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UDP_ECOLI UDP_ECOLI] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lx/1lx7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lx7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LX7 OCA].
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*[[Uridine phosphorylase 3D structures|Uridine phosphorylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of Escherichia coli uridine phosphorylase at 2.0 A., Burling FT, Kniewel R, Buglino JA, Chadha T, Beckwith A, Lima CD, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):73-6. Epub 2002 Dec, 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12499542 12499542]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Uridine phosphorylase]]
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[[Category: Beckwith A]]
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[[Category: Beckwith, A.]]
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[[Category: Buglino JA]]
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[[Category: Buglino, J A.]]
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[[Category: Burley SK]]
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[[Category: Burley, S K.]]
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[[Category: Burling T]]
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[[Category: Burling, T.]]
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[[Category: Chadna T]]
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[[Category: Chadna, T.]]
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[[Category: Kniewel R]]
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[[Category: Kniewel, R.]]
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[[Category: Lima CD]]
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[[Category: Lima, C D.]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nucleotide metabolism]]
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[[Category: nysgxrc]]
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[[Category: p12758]]
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[[Category: phosphorylase]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: udrpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:20 2008''
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Current revision

Structure of E. coli uridine phosphorylase at 2.0A

PDB ID 1lx7

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