2j8t

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[[Image:2j8t.png|left|200px]]
 
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{{STRUCTURE_2j8t| PDB=2j8t | SCENE= }}
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==Human aldose reductase in complex with NADP and citrate at 0.82 angstrom==
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<StructureSection load='2j8t' size='340' side='right'caption='[[2j8t]], [[Resolution|resolution]] 0.82&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J8T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.82&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j8t OCA], [https://pdbe.org/2j8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j8t RCSB], [https://www.ebi.ac.uk/pdbsum/2j8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j8t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALDR_HUMAN ALDR_HUMAN] Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/2j8t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j8t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of human aldose reductase in complex with citrate has been determined to a resolution of 0.82 A. The difference electron density for H atoms unequivocally shows that the cofactor is in the oxidized state corresponding to the situation after the catalytic event has occurred. A citrate molecule bound to the active site has been modelled in two different conformations. These two conformations correlate with a fully closed and a partially open conformation of the so-called safety-belt loop (Gly213-Ser226). The open conformation is observed for the first time with the cofactor bound to the protein and may be related to the initial phase of the opening of the safety belt. The structure suggests that after the catalytic event, a rearrangement of a bound ligand can trigger the opening of the safety-belt loop, thus initiating the release of the oxidized cofactor.
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===HUMAN ALDOSE REDUCTASE IN COMPLEX WITH NADP AND CITRATE AT 0.82 ANGSTROM===
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The atomic resolution structure of human aldose reductase reveals that rearrangement of a bound ligand allows the opening of the safety-belt loop.,Biadene M, Hazemann I, Cousido A, Ginell S, Joachimiak A, Sheldrick GM, Podjarny A, Schneider TR Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):665-72. Epub 2007, May 15. PMID:17505104<ref>PMID:17505104</ref>
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{{ABSTRACT_PUBMED_17505104}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2j8t" style="background-color:#fffaf0;"></div>
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[[2j8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J8T OCA].
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== References ==
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<references/>
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==See Also==
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__TOC__
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*[[Aldose Reductase|Aldose Reductase]]
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:017505104</ref><references group="xtra"/>
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[[Category: Aldehyde reductase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Biadene, M.]]
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[[Category: Large Structures]]
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[[Category: Cousido, A.]]
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[[Category: Biadene M]]
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[[Category: Ginell, S.]]
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[[Category: Cousido A]]
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[[Category: Hazemann, I.]]
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[[Category: Ginell S]]
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[[Category: Podjarny, A.]]
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[[Category: Hazemann I]]
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[[Category: Schneider, T R.]]
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[[Category: Podjarny A]]
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[[Category: Sheldrick, G M.]]
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[[Category: Schneider TR]]
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[[Category: Citrate]]
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[[Category: Sheldrick GM]]
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[[Category: Nadp]]
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[[Category: Oxidoreductase]]
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Current revision

Human aldose reductase in complex with NADP and citrate at 0.82 angstrom

PDB ID 2j8t

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