1q57

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:13, 14 February 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1q57.png|left|200px]]
 
-
{{STRUCTURE_1q57| PDB=1q57 | SCENE= }}
+
==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==
-
 
+
<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
-
===The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7===
+
== Structural highlights ==
-
 
+
<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
-
{{ABSTRACT_PUBMED_14636571}}
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [https://pdbe.org/1q57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [https://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>
-
==About this Structure==
+
</table>
-
[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA].
+
== Function ==
 +
[https://www.uniprot.org/uniprot/HELIC_BPT7 HELIC_BPT7] ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination (PubMed:21606333, PubMed:22977246, PubMed:32009150). The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis (PubMed:21606333, PubMed:22977246, PubMed:32009150). ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA (PubMed:30679383, PubMed:17604719). Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange (PubMed:9096333, PubMed:8617248). Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present (PubMed:6454135, PubMed:9139692).[HAMAP-Rule:MF_04154]<ref>PMID:17604719</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref> <ref>PMID:30679383</ref> <ref>PMID:32009150</ref> <ref>PMID:6454135</ref> <ref>PMID:8617248</ref> <ref>PMID:9096333</ref> <ref>PMID:9139692</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q57_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q57 ConSurf].
 +
<div style="clear:both"></div>
==See Also==
==See Also==
-
*[[RNA polymerase|RNA polymerase]]
+
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:014636571</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Enterobacteria phage t7]]
+
</StructureSection>
-
[[Category: Cheng, Y.]]
+
[[Category: Escherichia phage T7]]
-
[[Category: Ellenberger, T.]]
+
[[Category: Large Structures]]
-
[[Category: Li, Y.]]
+
[[Category: Cheng Y]]
-
[[Category: Sawaya, M R.]]
+
[[Category: Ellenberger T]]
-
[[Category: Toth, E A.]]
+
[[Category: Li Y]]
-
[[Category: Dna replication]]
+
[[Category: Sawaya MR]]
-
[[Category: Dntpase]]
+
[[Category: Toth EA]]
-
[[Category: Helicase]]
+
-
[[Category: Primase]]
+
-
[[Category: Transferase]]
+

Current revision

The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7

PDB ID 1q57

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1q57"
Personal tools