3fwl

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[[Image:3fwl.png|left|200px]]
 
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{{STRUCTURE_3fwl| PDB=3fwl | SCENE= }}
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==Crystal Structure of the Full-Length Transglycosylase PBP1b from Escherichia coli==
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<StructureSection load='3fwl' size='340' side='right'caption='[[3fwl]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FWL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.086&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M0E:MOENOMYCIN'>M0E</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fwl OCA], [https://pdbe.org/3fwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fwl RCSB], [https://www.ebi.ac.uk/pdbsum/3fwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fwl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PBPB_ECOLI PBPB_ECOLI] Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/3fwl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fwl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Drug-resistant bacteria have caused serious medical problems in recent years, and the need for new antibacterial agents is undisputed. Transglycosylase, a multidomain membrane protein essential for cell wall synthesis, is an excellent target for the development of new antibiotics. Here, we determined the X-ray crystal structure of the bifunctional transglycosylase penicillin-binding protein 1b (PBP1b) from Escherichia coli in complex with its inhibitor moenomycin to 2.16-A resolution. In addition to the transglycosylase and transpeptidase domains, our structure provides a complete visualization of this important antibacterial target, and reveals a domain for protein-protein interaction and a transmembrane helix domain essential for substrate binding, enzymatic activity, and membrane orientation.
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===Crystal Structure of the Full-Length Transglycosylase PBP1b from Escherichia coli===
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Crystal structure of the membrane-bound bifunctional transglycosylase PBP1b from Escherichia coli.,Sung MT, Lai YT, Huang CY, Chou LY, Shih HW, Cheng WC, Wong CH, Ma C Proc Natl Acad Sci U S A. 2009 May 19. PMID:19458048<ref>PMID:19458048</ref>
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{{ABSTRACT_PUBMED_19458048}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3fwl" style="background-color:#fffaf0;"></div>
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[[3fwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FWL OCA].
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==See Also==
==See Also==
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*[[Penicillin-binding protein|Penicillin-binding protein]]
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*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019458048</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Chou, L Y.]]
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[[Category: Large Structures]]
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[[Category: Huang, C Y.]]
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[[Category: Chou LY]]
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[[Category: Lai, Y T.]]
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[[Category: Huang CY]]
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[[Category: Ma, C.]]
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[[Category: Lai YT]]
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[[Category: Sung, M T.]]
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[[Category: Ma C]]
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[[Category: Wong, C H.]]
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[[Category: Sung MT]]
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[[Category: Alternative initiation]]
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[[Category: Wong CH]]
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[[Category: Antibiotic resistance]]
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[[Category: Antibiotics design]]
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[[Category: Bacterial cell wall synthesis]]
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[[Category: Cell inner membrane]]
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[[Category: Cell membrane]]
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[[Category: Cell shape]]
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[[Category: Cell wall biogenesis/degradation]]
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[[Category: Glycosyltransferase]]
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[[Category: Hydrolase]]
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[[Category: Membrane]]
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[[Category: Multifunctional enzyme]]
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[[Category: Penicillin-binding protein]]
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[[Category: Peptidoglycan synthesis]]
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[[Category: Signal-anchor]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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Current revision

Crystal Structure of the Full-Length Transglycosylase PBP1b from Escherichia coli

PDB ID 3fwl

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