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3sq5
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| - | [[Image:3sq5.png|left|200px]] | ||
| - | + | ==Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H432N Mutant== | |
| - | + | <StructureSection load='3sq5' size='340' side='right'caption='[[3sq5]], [[Resolution|resolution]] 2.30Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3sq5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SQ5 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sq5 OCA], [https://pdbe.org/3sq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sq5 RCSB], [https://www.ebi.ac.uk/pdbsum/3sq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sq5 ProSAT]</span></td></tr> | |
| - | == | + | </table> |
| - | [[3sq5]] is a 4 chain structure with sequence from [ | + | == Function == |
| + | [https://www.uniprot.org/uniprot/TYDP1_YEAST TYDP1_YEAST] DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Cleaves also 5' phosphotyrosyl adducts resulting from dead-end complexes between DNA and the active site tyrosine of topoisomerase II. Contributes to DNA repair after radiation damage. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).<ref>PMID:10521354</ref> <ref>PMID:16751265</ref> | ||
==See Also== | ==See Also== | ||
| - | *[[Phosphodiesterase|Phosphodiesterase]] | + | *[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: | + | [[Category: Gajewski S]] |
| - | + | [[Category: White SW]] | |
| - | + | ||
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Current revision
Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H432N Mutant
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