1mg4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:43, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mg4.jpg|left|200px]]<br /><applet load="1mg4" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1mg4, resolution 1.504&Aring;" />
 
-
'''STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN'''<br />
 
-
==Overview==
+
==STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN==
-
The doublecortin-like domains (DCX), which typically occur in tandem, are novel microtubule-binding modules. DCX tandems are found in doublecortin, a 360-residue protein expressed in migrating neurons; the doublecortin-like kinase (DCLK); the product of the RP1 gene that is responsible for a form of inherited blindness; and several other proteins. Mutations in the gene encoding doublecortin cause lissencephaly in males and the 'double-cortex syndrome' in females. We here report a solution structure of the N-terminal DCX domain of human doublecortin and a 1.5 A resolution crystal structure of the equivalent domain from human DCLK. Both show a stable, ubiquitin-like tertiary fold with distinct structural similarities to GTPase-binding domains. We also show that the C-terminal DCX domains of both proteins are only partially folded. In functional assays, the N-terminal DCX domain of doublecortin binds only to assembled microtubules, whereas the C-terminal domain binds to both microtubules and unpolymerized tubulin.
+
<StructureSection load='1mg4' size='340' side='right'caption='[[1mg4]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1mg4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MG4 FirstGlance]. <br>
-
1MG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MG4 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.504&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mg4 OCA], [https://pdbe.org/1mg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mg4 RCSB], [https://www.ebi.ac.uk/pdbsum/1mg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mg4 ProSAT]</span></td></tr>
-
The DCX-domain tandems of doublecortin and doublecortin-like kinase., Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH, Derewenda ZS, Nat Struct Biol. 2003 May;10(5):324-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12692530 12692530]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DCLK1_HUMAN DCLK1_HUMAN] Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mg4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mg4 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bushweller, J H.]]
+
[[Category: Bushweller JH]]
-
[[Category: Cierpickil, T.]]
+
[[Category: Cierpickil T]]
-
[[Category: Dauter, Z.]]
+
[[Category: Dauter Z]]
-
[[Category: Derewenda, U.]]
+
[[Category: Derewenda U]]
-
[[Category: Derewenda, Z.]]
+
[[Category: Derewenda Z]]
-
[[Category: Devedjiev, Y.]]
+
[[Category: Devedjiev Y]]
-
[[Category: Feng, Y.]]
+
[[Category: Feng Y]]
-
[[Category: Kim, M H.]]
+
[[Category: Kim MH]]
-
[[Category: Krowarsch, D.]]
+
[[Category: Krowarsch D]]
-
[[Category: Otlewski, J.]]
+
[[Category: Otlewski J]]
-
[[Category: Walsh, C A.]]
+
[[Category: Walsh CA]]
-
[[Category: SO4]]
+
-
[[Category: cortex development]]
+
-
[[Category: dcx domain]]
+
-
[[Category: doublecortin-like kinase]]
+
-
[[Category: microtubule bundling]]
+
-
[[Category: x-ray structure]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:08 2008''
+

Current revision

STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN

PDB ID 1mg4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mg4"
Personal tools