1mhq

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[[Image:1mhq.jpg|left|200px]]<br /><applet load="1mhq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mhq, resolution 2.2&Aring;" />
 
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'''Crystal Structure Of Human GGA2 VHS Domain'''<br />
 
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==Overview==
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==Crystal Structure Of Human GGA2 VHS Domain==
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Golgi-localized, gamma-ear-containing, ARF binding (GGA) proteins regulate intracellular vesicle transport by recognizing sorting signals on the cargo surface in the initial step of the budding process. The VHS (VPS27, Hrs, and STAM) domain of GGA binds with the signal peptides. Here, a crystal structure of the VHS domain of GGA2 is reported at 2.2 A resolution, which permits a direct comparison with that of homologous proteins, GGA1 and GGA3. Significant structural difference is present in the loop between helices 6 and 7, which forms part of the ligand binding pocket. Intrinsic fluorescence spectroscopic study indicates that this loop undergoes a conformational change upon ligand binding. Thus, the current structure suggests that a conformational change induced by ligand binding occurs in this part of the ligand pocket.
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<StructureSection load='1mhq' size='340' side='right'caption='[[1mhq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mhq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MHQ FirstGlance]. <br>
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1MHQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mhq OCA], [https://pdbe.org/1mhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mhq RCSB], [https://www.ebi.ac.uk/pdbsum/1mhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mhq ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of GGA2 VHS domain and its implication in plasticity in the ligand binding pocket., Zhu G, He X, Zhai P, Terzyan S, Tang J, Zhang XC, FEBS Lett. 2003 Feb 27;537(1-3):171-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12606052 12606052]
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== Function ==
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[https://www.uniprot.org/uniprot/GGA2_HUMAN GGA2_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/1mhq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mhq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Zhang, X C.]]
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[[Category: Zhang XC]]
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[[Category: Zhu, G.]]
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[[Category: Zhu G]]
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[[Category: super helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:19 2008''
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Current revision

Crystal Structure Of Human GGA2 VHS Domain

PDB ID 1mhq

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