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1mi7

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[[Image:1mi7.gif|left|200px]]<br /><applet load="1mi7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mi7, resolution 2.50&Aring;" />
 
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'''Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol'''<br />
 
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==Overview==
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==Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol==
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The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.
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<StructureSection load='1mi7' size='340' side='right'caption='[[1mi7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI7 FirstGlance]. <br>
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1MI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [https://pdbe.org/1mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi7 ProSAT]</span></td></tr>
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E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274929 15274929]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRPR_ECOLI TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mi7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Benoff, B.]]
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[[Category: Benoff B]]
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[[Category: Berger, T.]]
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[[Category: Berger T]]
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[[Category: Berman, H M.]]
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[[Category: Berman HM]]
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[[Category: Carey, J.]]
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[[Category: Carey J]]
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[[Category: Lawson, C L.]]
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[[Category: Lawson CL]]
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[[Category: IPA]]
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[[Category: alcohol induced conformational rearrangement]]
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[[Category: dna binding protein]]
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[[Category: domain swapping]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:25 2008''
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Current revision

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

PDB ID 1mi7

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