1mkj

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[[Image:1mkj.gif|left|200px]]<br /><applet load="1mkj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mkj, resolution 2.70&Aring;" />
 
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'''Human Kinesin Motor Domain With Docked Neck Linker'''<br />
 
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==Overview==
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==Human Kinesin Motor Domain With Docked Neck Linker==
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Crystal structures of the molecular motor kinesin show conformational variability in a structural element called the neck linker. Conformational change in the neck linker, initiated by ATP exchange, is thought to drive the movement of kinesin along the microtubule track. We use site-specific EPR measurements to show that when microtubules are absent, the neck linker exists in equilibrium between two structural states (disordered and 'docked'). The active site nucleotide does not control the position taken by the neck linker. However, we find that sulfate can specifically bind near the nucleotide site and stabilize the docked neck linker conformation, which we confirmed by solving a new crystal structure. Comparing the crystal structures of our construct with the docked or undocked neck linker reveals how microtubule binding may activate the nucleotide-sensing mechanism of kinesin, allowing neck linker transitions to power motility.
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<StructureSection load='1mkj' size='340' side='right'caption='[[1mkj]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MKJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mkj OCA], [https://pdbe.org/1mkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mkj RCSB], [https://www.ebi.ac.uk/pdbsum/1mkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mkj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mkj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mkj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKJ OCA].
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy., Sindelar CV, Budny MJ, Rice S, Naber N, Fletterick R, Cooke R, Nat Struct Biol. 2002 Nov;9(11):844-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12368902 12368902]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Budny, M J.]]
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[[Category: Budny MJ]]
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[[Category: Cooke, R.]]
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[[Category: Cooke R]]
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[[Category: Fletterick, R.]]
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[[Category: Fletterick R]]
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[[Category: Naber, N.]]
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[[Category: Naber N]]
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[[Category: Rice, S.]]
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[[Category: Rice S]]
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[[Category: Sindelar, C V.]]
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[[Category: Sindelar CV]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: atpase]]
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[[Category: microtubule]]
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[[Category: motor protein]]
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[[Category: myosin]]
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[[Category: neck linker]]
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[[Category: relay helix]]
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[[Category: switch ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:07 2008''
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Current revision

Human Kinesin Motor Domain With Docked Neck Linker

PDB ID 1mkj

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