2wgz

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[[Image:2wgz.png|left|200px]]
 
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{{STRUCTURE_2wgz| PDB=2wgz | SCENE= }}
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==Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)==
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<StructureSection load='2wgz' size='340' side='right'caption='[[2wgz]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [https://pdbe.org/2wgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [https://www.ebi.ac.uk/pdbsum/2wgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wgz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic.
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===CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (ALPHA3GT) IN A COMPLEX WITH P-NITROPHENYL-BETA-GALACTOSIDE (PNP-BETA-GAL)===
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Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884<ref>PMID:19486884</ref>
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{{ABSTRACT_PUBMED_19486884}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2wgz" style="background-color:#fffaf0;"></div>
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[[2wgz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA].
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==See Also==
==See Also==
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*[[Galactosyltransferase|Galactosyltransferase]]
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019486884</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Acharya, K R.]]
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[[Category: Large Structures]]
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[[Category: Brew, K.]]
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[[Category: Acharya KR]]
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[[Category: Ferns, T A.]]
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[[Category: Brew K]]
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[[Category: Jamaluddin, H.]]
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[[Category: Ferns TA]]
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[[Category: Thiyagarajan, N.]]
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[[Category: Jamaluddin H]]
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[[Category: Tumbale, P.]]
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[[Category: Thiyagarajan N]]
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[[Category: Alpha-1]]
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[[Category: Tumbale P]]
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[[Category: Enzyme kinetic]]
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[[Category: Galactosyltransferase]]
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[[Category: Glycoprotein]]
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[[Category: Glycosyltransferase]]
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[[Category: Golgi apparatus]]
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[[Category: Manganese]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Signal-anchor]]
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[[Category: Substrate binding]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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Current revision

Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)

PDB ID 2wgz

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OCA

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