2gmi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:08, 6 November 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2gmi.png|left|200px]]
 
-
{{STRUCTURE_2gmi| PDB=2gmi | SCENE= }}
+
==Mms2/Ubc13~Ubiquitin==
 +
<StructureSection load='2gmi' size='340' side='right'caption='[[2gmi]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2gmi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMI FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmi OCA], [https://pdbe.org/2gmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmi RCSB], [https://www.ebi.ac.uk/pdbsum/2gmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmi ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/UBC13_YEAST UBC13_YEAST] Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/2gmi_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gmi ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Lys63-linked polyubiquitin chains participate in nonproteolytic signaling pathways, including regulation of DNA damage tolerance and NF-kappaB activation. E2 enzymes bound to ubiquitin E2 variants (UEV) are vital in these pathways, synthesizing Lys63-linked polyubiquitin chains, but how these complexes achieve specificity for a particular lysine linkage has been unclear. We have determined the crystal structure of an Mms2-Ubc13-ubiquitin (UEV-E2-Ub) covalent intermediate with donor ubiquitin linked to the active site residue of Ubc13. In the structure, the unexpected binding of a donor ubiquitin of one Mms2-Ubc13-Ub complex to the acceptor-binding site of Mms2-Ubc13 in an adjacent complex allows us to visualize at atomic resolution the molecular determinants of acceptor-ubiquitin binding. The structure reveals the key role of Mms2 in allowing selective insertion of Lys63 into the Ubc13 active site and suggests a molecular model for polyubiquitin chain elongation.
-
===Mms2/Ubc13~Ubiquitin===
+
Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation.,Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C Nat Struct Mol Biol. 2006 Oct;13(10):915-20. Epub 2006 Sep 17. PMID:16980971<ref>PMID:16980971</ref>
-
{{ABSTRACT_PUBMED_16980971}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2gmi" style="background-color:#fffaf0;"></div>
-
[[2gmi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMI OCA].
+
==See Also==
==See Also==
-
*[[Ubiquitin|Ubiquitin]]
+
*[[3D structures of ubiquitin|3D structures of ubiquitin]]
-
 
+
*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
-
==Reference==
+
== References ==
-
<ref group="xtra">PMID:016980971</ref><ref group="xtra">PMID:017050691</ref><references group="xtra"/>
+
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Ubiquitin--protein ligase]]
+
[[Category: Carlile CM]]
-
[[Category: Carlile, C M.]]
+
[[Category: Eddins MJ]]
-
[[Category: Eddins, M J.]]
+
[[Category: Gomez KG]]
-
[[Category: Gomez, K G.]]
+
[[Category: Pickart CM]]
-
[[Category: Pickart, C M.]]
+
[[Category: Wolberger C]]
-
[[Category: Wolberger, C.]]
+
-
[[Category: E2]]
+
-
[[Category: Human protein]]
+
-
[[Category: Ligase]]
+
-
[[Category: Mms2]]
+
-
[[Category: Ubc13]]
+
-
[[Category: Ubc13 ubiquitin covalent complex]]
+
-
[[Category: Ubiquitin]]
+
-
[[Category: Uev]]
+

Current revision

Mms2/Ubc13~Ubiquitin

PDB ID 2gmi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gmi"
Personal tools