1moy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:46, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1moy.gif|left|200px]]<br /><applet load="1moy" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1moy, resolution 1.55&Aring;" />
 
-
'''Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD'''<br />
 
-
==Overview==
+
==Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD==
-
The RGD (arginine-glycine-aspartic acid) sequence is found in several important extracellular matrix proteins and serves as an adhesion ligand for members of the integrin family of cell-surface receptors. This sequence and flanking residues from fibronectin or osteopontin have been engineered into an accessible surface loop of streptavidin to create two new streptavidin variants (FN-SA or OPN-SA, respectively) that bind cells through the alpha(v)beta(3) and/or alpha(5)beta(1) integrin receptors. Their crystal structures confirm the design and construction of the mutants and provide evidence about the conformational dynamics of the RGD loops. The loops in the isomorphous crystal structures are involved in crystal-packing interactions and this stabilizes their structures. Even so, the loop in OPN-SA is slightly disordered and two of the residues are not seen in difference electron-density maps. Comparison with other experimentally determined structures of RGD loops in cell-adhesion molecules shows that these loops occupy a large subset of conformational space. This is consistent with the view that RGD loops, at least those involved in cell adhesion, sample a number of structures dynamically, a few of which display high affinity for appropriate receptors.
+
<StructureSection load='1moy' size='340' side='right'caption='[[1moy]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1moy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOY FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1moy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1moy OCA], [https://pdbe.org/1moy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1moy RCSB], [https://www.ebi.ac.uk/pdbsum/1moy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1moy ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1moy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1moy ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1MOY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOY OCA].
+
*[[Avidin 3D structures|Avidin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin., Le Trong I, McDevitt TC, Nelson KE, Stayton PS, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):828-34. Epub 2003, Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12777798 12777798]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
-
[[Category: McDevitt, T C.]]
+
[[Category: Le Trong I]]
-
[[Category: Nelson, K E.]]
+
[[Category: McDevitt TC]]
-
[[Category: Stayton, P S.]]
+
[[Category: Nelson KE]]
-
[[Category: Stenkamp, R E.]]
+
[[Category: Stayton PS]]
-
[[Category: Trong, I Le.]]
+
[[Category: Stenkamp RE]]
-
[[Category: MRD]]
+
-
[[Category: tetramer]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:27 2008''
+

Current revision

Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD

PDB ID 1moy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1moy"
Personal tools