1yit

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[[Image:1yit.png|left|200px]]
 
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{{STRUCTURE_1yit| PDB=1yit | SCENE= }}
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==Crystal Structure Of Virginiamycin M and S Bound To The 50S Ribosomal Subunit Of Haloarcula Marismortui==
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<StructureSection load='1yit' size='340' side='right'caption='[[1yit]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1yit]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] and [https://en.wikipedia.org/wiki/Streptomyces_virginiae Streptomyces virginiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YIT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=004:(2S)-AMINO(PHENYL)ETHANOIC+ACID'>004</scene>, <scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MHV:4-OXO-L-PIPECOLIC+ACID'>MHV</scene>, <scene name='pdbligand=MHW:3-HYDROXYPICOLINIC+ACID'>MHW</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=VIR:VIRGINIAMYCIN+M1'>VIR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yit OCA], [https://pdbe.org/1yit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yit RCSB], [https://www.ebi.ac.uk/pdbsum/1yit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yit ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL44E_HALMA RL44E_HALMA] Binds to the 23S rRNA. Binds deacetylated tRNA in the E site; when the tRNA binds a stretch of 7 amino acids are displaced to allow binding.[HAMAP-Rule:MF_01476]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yi/1yit_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yit ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of H. marismortui large ribosomal subunits containing the mutation G2099A (A2058 in E. coli) with erythromycin, azithromycin, clindamycin, virginiamycin S, and telithromycin bound explain why eubacterial ribosomes containing the mutation A2058G are resistant to them. Azithromycin binds almost identically to both G2099A and wild-type subunits, but the erythromycin affinity increases by more than 10(4)-fold, implying that desolvation of the N2 of G2099 accounts for the low wild-type affinity for macrolides. All macrolides bind similarly to the H. marismortui subunit, but their binding differs significantly from what has been reported in the D. radioidurans subunit. The synergy in the binding of streptogramins A and B appears to result from a reorientation of the base of A2103 (A2062, E. coli) that stacks between them. The structure of large subunit containing a three residue deletion mutant of L22 shows a change in the L22 structure and exit tunnel shape that illuminates its macrolide resistance phenotype.
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===Crystal Structure Of Virginiamycin M and S Bound To The 50S Ribosomal Subunit Of Haloarcula Marismortui===
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Structures of MLSBK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance.,Tu D, Blaha G, Moore PB, Steitz TA Cell. 2005 Apr 22;121(2):257-70. PMID:15851032<ref>PMID:15851032</ref>
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{{ABSTRACT_PUBMED_15851032}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1yit" style="background-color:#fffaf0;"></div>
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[[1yit]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] and [http://en.wikipedia.org/wiki/Streptomyces_virginiae Streptomyces virginiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YIT OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein L10|Ribosomal protein L10]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein L11|Ribosomal protein L11]]
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== References ==
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*[[Ribosomal protein L13|Ribosomal protein L13]]
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<references/>
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*[[Ribosomal protein L14|Ribosomal protein L14]]
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__TOC__
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*[[Ribosomal protein L2|Ribosomal protein L2]]
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</StructureSection>
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*[[Ribosomal protein L3|Ribosomal protein L3]]
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*[[Ribosomal protein L5|Ribosomal protein L5]]
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*[[Ribosomal protein L6|Ribosomal protein L6]]
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*[[Ribosomal protein L7|Ribosomal protein L7]]
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*[[Ribosome|Ribosome]]
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==Reference==
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<ref group="xtra">PMID:015851032</ref><references group="xtra"/>
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[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
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[[Category: Large Structures]]
[[Category: Streptomyces virginiae]]
[[Category: Streptomyces virginiae]]
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[[Category: Blaha, G.]]
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[[Category: Blaha G]]
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[[Category: Moore, P B.]]
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[[Category: Moore PB]]
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[[Category: Steitz, T A.]]
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[[Category: Steitz TA]]
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[[Category: Tu, D.]]
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[[Category: Tu D]]
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[[Category: Antibiotic]]
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[[Category: Cyclohexadepsipeptide]]
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[[Category: Peptidyl transferase]]
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[[Category: Ribosome]]
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[[Category: Ribosome-antibiotic complex]]
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[[Category: Streptogramin]]
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[[Category: Virginiamycin m]]
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[[Category: Virginiamycin s]]
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Current revision

Crystal Structure Of Virginiamycin M and S Bound To The 50S Ribosomal Subunit Of Haloarcula Marismortui

PDB ID 1yit

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