2bv3

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[[Image:2bv3.png|left|200px]]
 
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{{STRUCTURE_2bv3| PDB=2bv3 | SCENE= }}
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==Crystal structure of a mutant elongation factor G trapped with a GTP analogue==
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<StructureSection load='2bv3' size='340' side='right'caption='[[2bv3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BV3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bv3 OCA], [https://pdbe.org/2bv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bv3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bv3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EFG_THETH EFG_THETH] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bv/2bv3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bv3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Elongation factor G (EF-G) is a G protein factor that catalyzes the translocation step in protein synthesis on the ribosome. Its GTP conformation in the absence of the ribosome is currently unknown. We present the structure of a mutant EF-G (T84A) in complex with the non-hydrolysable GTP analogue GDPNP. The crystal structure provides a first insight into conformational changes induced in EF-G by GTP. Comparison of this structure with that of EF-G in complex with GDP suggests that the GTP and GDP conformations in solution are very similar and that the major contribution to the active GTPase conformation, which is quite different, therefore comes from its interaction with the ribosome.
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===CRYSTAL STRUCTURE OF A MUTANT ELONGATION FACTOR G TRAPPED WITH A GTP ANALOGUE===
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Crystal structure of a mutant elongation factor G trapped with a GTP analogue.,Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:16083884<ref>PMID:16083884</ref>
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{{ABSTRACT_PUBMED_16083884}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2bv3" style="background-color:#fffaf0;"></div>
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[[2bv3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BV3 OCA].
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==See Also==
==See Also==
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*[[Elongation factor|Elongation factor]]
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016083884</ref><ref group="xtra">PMID:008070396</ref><ref group="xtra">PMID:011054294</ref><ref group="xtra">PMID:011371559</ref><ref group="xtra">PMID:015843024</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Gudkov, A T.]]
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[[Category: Gudkov AT]]
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[[Category: Hansson, S.]]
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[[Category: Hansson S]]
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[[Category: Liljas, A.]]
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[[Category: Liljas A]]
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[[Category: Logan, D T.]]
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[[Category: Logan DT]]
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[[Category: Singh, R.]]
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[[Category: Singh R]]
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[[Category: Elongation factor]]
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[[Category: Gtp-binding]]
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[[Category: Protein biosynthesis]]
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[[Category: Switch ii]]
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[[Category: Translation mutation thr84ala]]
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Current revision

Crystal structure of a mutant elongation factor G trapped with a GTP analogue

PDB ID 2bv3

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