3jrv

From Proteopedia

(Difference between revisions)

Current revision

Structure of poxvirus K7 protein in complex with RNA helicase DDX3

Structural highlights

3jrv is a 5 chain structure with sequence from Homo sapiens and Vaccinia virus WR. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PG044_VACCW Virulence factor that affects the acute immune response to infection (PubMed:23580427). Bcl-2-like protein which, through its interaction with the DEAD box RNA helicase DDX3X/DDX3, prevents TBK1/IKKepsilon-mediated IRF3 activation. Contributes to virulence by binding to the host TRAF6 and IRAK2 and preventing host NF-kappa-B activation.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Poxviruses are DNA viruses that express numerous proteins to subvert the host immune response. Vaccinia virus protein K7 adopts a Bcl-2 fold and displays structural and functional similarities to Toll-like receptor antagonist A52. Both proteins interact with IRAK2 and TRAF6 and suppress TLR-dependent NF-kappaB activation. However, unlike A52, K7 also forms a complex with RNA helicase DDX3 and antagonizes interferon-beta promoter induction. We have narrowed the K7 binding site to an N-terminal peptide motif of DDX3 ahead of its core RNA-helicase domains. The crystal structure of full-length K7 in complex with the DDX3 peptide reveals a thumblike projection of tandem phenalyalanine residues of DDX3 into a deep hydrophobic cleft. Mutagenesis of these phenylalanines abolishes the effects of DDX3 on interferon-beta promoter induction. The structure of K7-DDX3 reveals a novel binding mode by a viral Bcl-2 protein that antagonizes a key pathway in innate immunity.

Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7.,Oda S, Schroder M, Khan AR Structure. 2009 Nov 11;17(11):1528-37. PMID:19913487^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schroder M, Baran M, Bowie AG. Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation. EMBO J. 2008 Aug 6;27(15):2147-57. doi: 10.1038/emboj.2008.143. Epub 2008 Jul 17. PMID:18636090 doi:http://dx.doi.org/10.1038/emboj.2008.143
↑ Oda S, Schroder M, Khan AR. Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Structure. 2009 Nov 11;17(11):1528-37. PMID:19913487 doi:10.1016/j.str.2009.09.005
↑ Benfield CTO, Ren H, Lucas SJ, Bahsoun B, Smith GL. Vaccinia virus protein K7 is a virulence factor that alters the acute immune response to infection. J Gen Virol. 2013 Jul;94(Pt 7):1647-1657. PMID:23580427 doi:10.1099/vir.0.052670-0
↑ Oda S, Schroder M, Khan AR. Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Structure. 2009 Nov 11;17(11):1528-37. PMID:19913487 doi:10.1016/j.str.2009.09.005

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3jrv"

Categories: Homo sapiens | Large Structures | Vaccinia virus WR | Khan RA | Oda S

@@ Line 1: / Line 1: @@
-[[Image:3jrv.png|left|200px]]
-{{STRUCTURE_3jrv|  PDB=3jrv  |  SCENE=  }}
+==Structure of poxvirus K7 protein in complex with RNA helicase DDX3==
+<StructureSection load='3jrv' size='340' side='right'caption='[[3jrv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3jrv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JRV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jrv OCA], [https://pdbe.org/3jrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jrv RCSB], [https://www.ebi.ac.uk/pdbsum/3jrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jrv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PG044_VACCW PG044_VACCW] Virulence factor that affects the acute immune response to infection (PubMed:23580427). Bcl-2-like protein which, through its interaction with the DEAD box RNA helicase DDX3X/DDX3, prevents TBK1/IKKepsilon-mediated IRF3 activation. Contributes to virulence by binding to the host TRAF6 and IRAK2 and preventing host NF-kappa-B activation.<ref>PMID:18636090</ref> <ref>PMID:19913487</ref> <ref>PMID:23580427</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Poxviruses are DNA viruses that express numerous proteins to subvert the host immune response. Vaccinia virus protein K7 adopts a Bcl-2 fold and displays structural and functional similarities to Toll-like receptor antagonist A52. Both proteins interact with IRAK2 and TRAF6 and suppress TLR-dependent NF-kappaB activation. However, unlike A52, K7 also forms a complex with RNA helicase DDX3 and antagonizes interferon-beta promoter induction. We have narrowed the K7 binding site to an N-terminal peptide motif of DDX3 ahead of its core RNA-helicase domains. The crystal structure of full-length K7 in complex with the DDX3 peptide reveals a thumblike projection of tandem phenalyalanine residues of DDX3 into a deep hydrophobic cleft. Mutagenesis of these phenylalanines abolishes the effects of DDX3 on interferon-beta promoter induction. The structure of K7-DDX3 reveals a novel binding mode by a viral Bcl-2 protein that antagonizes a key pathway in innate immunity.
-===Structure of poxvirus K7 protein in complex with RNA helicase DDX3===
+Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7.,Oda S, Schroder M, Khan AR Structure. 2009 Nov 11;17(11):1528-37. PMID:19913487<ref>PMID:19913487</ref>
-{{ABSTRACT_PUBMED_19913487}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3jrv" style="background-color:#fffaf0;"></div>
-[[3jrv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus_wr Vaccinia virus wr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JRV OCA].
 ==See Also==
-*[[Helicase|Helicase]]
+*[[Helicase 3D structures|Helicase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019913487</ref><references group="xtra"/>
+__TOC__
-[[Category: Vaccinia virus wr]]
+</StructureSection>
-[[Category: Khan, R A.]]
+[[Category: Homo sapiens]]
-[[Category: Oda, S.]]
+[[Category: Large Structures]]
-[[Category: Dead-box rna helicase ddx3]]
+[[Category: Vaccinia virus WR]]
-[[Category: Innate immunity]]
+[[Category: Khan RA]]
-[[Category: Poxvirus protein k7]]
+[[Category: Oda S]]
-[[Category: Viral immune evasion]]
-[[Category: Viral protein-protein binding complex]]

3jrv

From Proteopedia

Current revision

Structure of poxvirus K7 protein in complex with RNA helicase DDX3

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox