1mse

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[[Image:1mse.gif|left|200px]]<br /><applet load="1mse" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mse" />
 
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'''SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES==
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<StructureSection load='1mse' size='340' side='right'caption='[[1mse]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mse]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mse OCA], [https://pdbe.org/1mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mse RCSB], [https://www.ebi.ac.uk/pdbsum/1mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mse ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYB_MOUSE MYB_MOUSE] Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1mse_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mse ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).
The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).
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==About this Structure==
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Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices.,Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kanai H, Sarai A, Ishii S, Nishimura Y Cell. 1994 Nov 18;79(4):639-48. PMID:7954830<ref>PMID:7954830</ref>
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1MSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices., Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kanai H, Sarai A, Ishii S, Nishimura Y, Cell. 1994 Nov 18;79(4):639-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7954830 7954830]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1mse" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Inoue, T.]]
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[[Category: Ishii, S.]]
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[[Category: Kanai, H.]]
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[[Category: Morikawa, S.]]
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[[Category: Nakamura, H.]]
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[[Category: Nishimura, Y.]]
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[[Category: Ogata, K.]]
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[[Category: Sarai, A.]]
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[[Category: Sekikawa, A.]]
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[[Category: c-myb dna-binding domain]]
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[[Category: dna]]
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[[Category: double helix]]
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[[Category: nmr]]
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[[Category: protooncogene product]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:34 2008''
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==See Also==
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Inoue T]]
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[[Category: Ishii S]]
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[[Category: Kanai H]]
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[[Category: Morikawa S]]
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[[Category: Nakamura H]]
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[[Category: Nishimura Y]]
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[[Category: Ogata K]]
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[[Category: Sarai A]]
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[[Category: Sekikawa A]]

Current revision

SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES

PDB ID 1mse

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