1msp

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[[Image:1msp.gif|left|200px]]<br /><applet load="1msp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1msp, resolution 2.5&Aring;" />
 
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'''MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6'''<br />
 
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==Overview==
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==MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6==
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We have determined the structure of the Ascaris major sperm protein (MSP) to 2.5 A resolution using X-ray crystallography. The MSP polypeptide chain has an immunoglobulin-like fold based on a seven-stranded beta sandwich. In two strands, cis-proline residues impart distinctive kinks, and overall the structure most closely resembles that of the N-terminal domain of the bacterial chaperonin, PapD. In the C2 crystal form which we have solved here, two MSP chains are tightly associated in the asymmetric unit and are related by a non-crystallographic 2-fold rotation axis. This arrangement almost certainly represents the MSP dimer that is present in solution. Additionally, the arrangement of two MSP dimers at one of the crystallographic 2-fold axes in the 215 A unit cell suggests a possible mode for the assembly of MSP into the filaments which promote cell movement. This dimer-dimer association is based on a beta sheet extension mechanism between adjoining MSP monomers which resembles the interaction between PapD and its protein substrate.
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<StructureSection load='1msp' size='340' side='right'caption='[[1msp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1msp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSP FirstGlance]. <br>
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1MSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1msp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msp OCA], [https://pdbe.org/1msp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1msp RCSB], [https://www.ebi.ac.uk/pdbsum/1msp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1msp ProSAT]</span></td></tr>
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==Reference==
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</table>
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2.5 A resolution crystal structure of the motile major sperm protein (MSP) of Ascaris suum., Bullock TL, Roberts TM, Stewart M, J Mol Biol. 1996 Oct 25;263(2):284-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8913307 8913307]
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== Function ==
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[https://www.uniprot.org/uniprot/MSP1_ASCSU MSP1_ASCSU] Central component in molecular interactions underlying sperm crawling. Forms an extensive filament system that extends from sperm villipoda, along the leading edge of the pseudopod.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msp ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Ascaris suum]]
[[Category: Ascaris suum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bullock, T L.]]
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[[Category: Bullock TL]]
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[[Category: Roberts, T M.]]
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[[Category: Roberts TM]]
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[[Category: Stewart, M.]]
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[[Category: Stewart M]]
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[[Category: cell motility protein]]
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[[Category: cytoskeletal protein]]
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[[Category: sperm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:39 2008''
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Current revision

MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6

PDB ID 1msp

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