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1mtp

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[[Image:1mtp.jpg|left|200px]]<br /><applet load="1mtp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mtp, resolution 1.5&Aring;" />
 
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'''The X-ray crystal structure of a serpin from a thermophilic prokaryote'''<br />
 
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==Overview==
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==The X-ray crystal structure of a serpin from a thermophilic prokaryote==
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Serpins utilize conformational change to inhibit target proteinases; the price paid for this conformational flexibility is that many undergo temperature-induced polymerization. Despite this thermolability, serpins are present in the genomes of thermophilic prokaryotes, and here we characterize the first such serpin, thermopin. Thermopin is a proteinase inhibitor and, in comparison with human alpha(1)-antitrypsin, possesses enhanced stability at 60 degrees C. The 1.5 A crystal structure reveals novel structural features in regions implicated in serpin folding and stability. Thermopin possesses a C-terminal "tail" that interacts with the top of the A beta sheet and plays an important role in the folding/unfolding of the molecule. These data provide evidence as to how this unusual serpin has adapted to fold and function in a heated environment.
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<StructureSection load='1mtp' size='340' side='right'caption='[[1mtp]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mtp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MTP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mtp OCA], [https://pdbe.org/1mtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mtp RCSB], [https://www.ebi.ac.uk/pdbsum/1mtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mtp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q47NK3_THEFY Q47NK3_THEFY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/1mtp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mtp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MTP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTP OCA].
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*[[Serpin 3D structures|Serpin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment., Irving JA, Cabrita LD, Rossjohn J, Pike RN, Bottomley SP, Whisstock JC, Structure. 2003 Apr;11(4):387-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12679017 12679017]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Thermobifida fusca]]
[[Category: Thermobifida fusca]]
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[[Category: Bottomley, S P.]]
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[[Category: Bottomley SP]]
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[[Category: Cabrita, L D.]]
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[[Category: Cabrita LD]]
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[[Category: Irving, J A.]]
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[[Category: Irving JA]]
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[[Category: Pike, R N.]]
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[[Category: Pike RN]]
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[[Category: Rossjohn, J.]]
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[[Category: Rossjohn J]]
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[[Category: Whisstock, J C.]]
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[[Category: Whisstock JC]]
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[[Category: protease inhibitor]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:53 2008''
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Current revision

The X-ray crystal structure of a serpin from a thermophilic prokaryote

PDB ID 1mtp

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