1mwg
From Proteopedia
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| - | [[Image:1mwg.jpg|left|200px]]<br /><applet load="1mwg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1mwg" /> | ||
| - | '''STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
| - | == | + | ==STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE== |
| + | <StructureSection load='1mwg' size='340' side='right'caption='[[1mwg]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1mwg]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwg OCA], [https://pdbe.org/1mwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwg RCSB], [https://www.ebi.ac.uk/pdbsum/1mwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities. | The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities. | ||
| - | + | Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach.,Wu M, Turner DH Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939<ref>PMID:8703939</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1mwg" style="background-color:#fffaf0;"></div> |
| - | [[Category: Santalucia | + | == References == |
| - | [[Category: Turner | + | <references/> |
| - | [[Category: Wu | + | __TOC__ |
| - | + | </StructureSection> | |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Santalucia J]] | |
| - | + | [[Category: Turner DH]] | |
| - | + | [[Category: Wu M]] | |
Current revision
STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE
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