1izj

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[[Image:1izj.png|left|200px]]
 
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{{STRUCTURE_1izj| PDB=1izj | SCENE= }}
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==Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a==
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<StructureSection load='1izj' size='340' side='right'caption='[[1izj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1izj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IZJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1izj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1izj OCA], [https://pdbe.org/1izj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1izj RCSB], [https://www.ebi.ac.uk/pdbsum/1izj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1izj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NEPU1_THEVU NEPU1_THEVU] Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Also hydrolyzes cyclodextrins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/1izj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1izj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thermoactinomyces vulgaris R-47 produces two alpha-amylases, TVAI and TVAII, differing in substrate specificity from each other. TVAI favors high-molecular-weight substrates like starch, and scarcely hydrolyzes cyclomaltooligosaccharides (cyclodextrins) with a small cavity. TVAII favors low-molecular-weight substrates like oligosaccharides, and can efficiently hydrolyze cyclodextrins with various sized cavities. To understand the relationship between the structure and substrate specificity of these enzymes, we precisely examined the roles of key residues for substrate recognition by X-ray structural and kinetic parameter analyses of mutant enzymes and successfully obtained mutants in which the substrate specificity of each enzyme is partially converted into that of another.
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===Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a===
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Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 alpha-amylases TVAI and TVAII by site-directed mutagenesis.,Ohtaki A, Iguchi A, Mizuno M, Tonozuka T, Sakano Y, Kamitori S Carbohydr Res. 2003 Jul 22;338(15):1553-8. PMID:12860426<ref>PMID:12860426</ref>
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{{ABSTRACT_PUBMED_12860426}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1izj" style="background-color:#fffaf0;"></div>
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[[1izj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZJ OCA].
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==See Also==
==See Also==
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*[[Alpha-Amylase|Alpha-Amylase]]
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:012860426</ref><references group="xtra"/>
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__TOC__
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[[Category: Alpha-amylase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermoactinomyces vulgaris]]
[[Category: Thermoactinomyces vulgaris]]
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[[Category: Iguchi, A.]]
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[[Category: Iguchi A]]
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[[Category: Kamitori, S.]]
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[[Category: Kamitori S]]
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[[Category: Mizuno, M.]]
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[[Category: Mizuno M]]
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[[Category: Ohtaki, A.]]
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[[Category: Ohtaki A]]
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[[Category: Sakano, Y.]]
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[[Category: Sakano Y]]
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[[Category: Tonozuka, T.]]
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[[Category: Tonozuka T]]
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[[Category: Alpha-beta barrele]]
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[[Category: Hydrolase]]
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Current revision

Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a

PDB ID 1izj

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