1z7h

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[[Image:1z7h.png|left|200px]]
 
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{{STRUCTURE_1z7h| PDB=1z7h | SCENE= }}
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==2.3 Angstrom crystal structure of tetanus neurotoxin light chain==
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<StructureSection load='1z7h' size='340' side='right'caption='[[1z7h]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z7h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z7H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7h OCA], [https://pdbe.org/1z7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7h RCSB], [https://www.ebi.ac.uk/pdbsum/1z7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/1z7h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z7h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group.
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===2.3 Angstrom crystal structure of tetanus neurotoxin light chain===
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2.3 A crystal structure of tetanus neurotoxin light chain.,Breidenbach MA, Brunger AT Biochemistry. 2005 May 24;44(20):7450-7. PMID:15895988<ref>PMID:15895988</ref>
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{{ABSTRACT_PUBMED_15895988}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1z7h" style="background-color:#fffaf0;"></div>
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[[1z7h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7H OCA].
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==See Also==
==See Also==
*[[Tetanus toxin|Tetanus toxin]]
*[[Tetanus toxin|Tetanus toxin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015895988</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Clostridium tetani]]
[[Category: Clostridium tetani]]
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[[Category: Tentoxilysin]]
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[[Category: Large Structures]]
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[[Category: Breidenbach, M A.]]
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[[Category: Breidenbach MA]]
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[[Category: Brunger, A T.]]
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[[Category: Brunger AT]]
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[[Category: Hydrolase]]
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[[Category: Metalloprotease]]
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[[Category: Neurotransmission]]
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[[Category: Snare]]
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[[Category: Tent]]
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[[Category: Tetanus]]
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Current revision

2.3 Angstrom crystal structure of tetanus neurotoxin light chain

PDB ID 1z7h

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