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1mzw

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[[Image:1mzw.gif|left|200px]]<br /><applet load="1mzw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mzw, resolution 2.0&Aring;" />
 
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'''Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide'''<br />
 
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==Overview==
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==Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide==
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The spliceosomal cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle, interacting with homologous sequences in the proteins U4/U6-60K and hPrp18 during pre-mRNA splicing. We determined the crystal structure of the complex comprising cyclophilin H and the cognate domain of U4/U6-60K. The 31 amino acid fragment of U4/U6-60K is bound to a region remote from the cyclophilin active site. Residues Ile118-Phe121 of U4/U6-60K expand the central beta-sheet of cyclophilin H and the side-chain of Phe121 inserts into a hydrophobic cavity. Concomitantly, in the crystal the cyclophilin H active site is occupied by the N terminus of a neighboring cyclophilin H molecule in a substrate-like manner, indicating the capacity of joint binding to a substrate and to U4/U6-60K. Free and complexed cyclophilin H have virtually identical conformations suggesting that the U4/U6-60K binding site is pre-shaped and the peptidyl-prolyl-cis/trans isomerase activity is unaffected by complex formation. The complex defines a novel protein-protein interaction mode for a cyclophilin, allowing cyclophilin H to mediate interactions between different proteins inside the spliceosome or to initiate from its binding platforms isomerization or chaperoning activities.
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<StructureSection load='1mzw' size='340' side='right'caption='[[1mzw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzw OCA], [https://pdbe.org/1mzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mzw RCSB], [https://www.ebi.ac.uk/pdbsum/1mzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPIH_HUMAN PPIH_HUMAN] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone.<ref>PMID:9570313</ref> <ref>PMID:11823439</ref> <ref>PMID:12875835</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mz/1mzw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mzw ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Cardiomyopathy, dilated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605906 605906]], Cardiomyopathy, dilated, with left ventricular noncompaction OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605906 605906]], Myopathy, myofibrillar, ZASP-related OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605906 605906]]
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*[[Cyclophilin 3D structures|Cyclophilin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1MZW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZW OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide., Reidt U, Wahl MC, Fasshauer D, Horowitz DS, Luhrmann R, Ficner R, J Mol Biol. 2003 Aug 1;331(1):45-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12875835 12875835]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ficner, R.]]
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[[Category: Ficner R]]
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[[Category: Horowitz, D S.]]
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[[Category: Horowitz DS]]
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[[Category: Luehrmann, R.]]
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[[Category: Luehrmann R]]
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[[Category: Reidt, U.]]
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[[Category: Reidt U]]
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[[Category: Wahl, M C.]]
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[[Category: Wahl MC]]
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[[Category: cyclophilin]]
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[[Category: peptidyl-prolyl-cis/trans isomerase]]
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[[Category: snrnp]]
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[[Category: spliceosome]]
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[[Category: u4/u6-60k protein]]
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[[Category: wd protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:47 2008''
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Current revision

Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide

PDB ID 1mzw

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