1b69

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[[Image:1b69.png|left|200px]]
 
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{{STRUCTURE_1b69| PDB=1b69 | SCENE= }}
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==THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX==
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<StructureSection load='1b69' size='340' side='right'caption='[[1b69]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1b69]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B69 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b69 OCA], [https://pdbe.org/1b69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b69 RCSB], [https://www.ebi.ac.uk/pdbsum/1b69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b69 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNR6_ENTFL TNR6_ENTFL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/1b69_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b69 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The integrase protein catalyzes the excision and integration of the Tn916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which the face of a three-stranded antiparallel beta-sheet is positioned within the major groove. A comparison to the structure of the homing endonuclease I-Ppol-DNA complex suggests that the three-stranded sheet may represent a new DNA-binding motif whose residue composition and position within the major groove are varied to alter specificity. The structure also provides insights into the mechanism of conjugative transposition. The DNA in the complex is bent approximately 35 degrees and may, together with potential interactions between bound integrase proteins at directly repeated sites, significantly bend the arms of the transposon.
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===THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX===
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NMR structure of the Tn916 integrase-DNA complex.,Wojciak JM, Connolly KM, Clubb RT Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:10201406<ref>PMID:10201406</ref>
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{{ABSTRACT_PUBMED_10201406}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1b69" style="background-color:#fffaf0;"></div>
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[[1b69]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B69 OCA].
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==See Also==
==See Also==
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*[[Retroviral Integrase|Retroviral Integrase]]
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:010201406</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
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[[Category: Clubb, R T.]]
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[[Category: Large Structures]]
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[[Category: Connolly, K M.]]
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[[Category: Synthetic construct]]
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[[Category: Wojciak, J M.]]
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[[Category: Clubb RT]]
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[[Category: Beta-sheet recognition]]
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[[Category: Connolly KM]]
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[[Category: Complex]]
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[[Category: Wojciak JM]]
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[[Category: Dna binding]]
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[[Category: Integrase]]
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[[Category: Integrase-dna complex]]
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[[Category: Transposition]]
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Current revision

THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX

PDB ID 1b69

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