1gq8
From Proteopedia
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| - | [[Image:1gq8.png|left|200px]] | ||
| - | + | ==Pectin methylesterase from Carrot== | |
| + | <StructureSection load='1gq8' size='340' side='right'caption='[[1gq8]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1gq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Daucus_carota Daucus carota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GQ8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gq8 OCA], [https://pdbe.org/1gq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gq8 RCSB], [https://www.ebi.ac.uk/pdbsum/1gq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gq8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PME_DAUCA PME_DAUCA] Catalyzes the deesterification of methyl-esterified D-galactosiduronic acid units in pectic compounds. It participates in modulating cell wall during fruit ripening, cell wall extension during pollen germination, and in defense mechanisms against pathogens. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/1gq8_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gq8 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The beta-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. | ||
| - | + | Crystal structure of plant pectin methylesterase.,Johansson K, El-Ahmad M, Friemann R, Jornvall H, Markovic O, Eklund H FEBS Lett. 2002 Mar 13;514(2-3):243-9. PMID:11943159<ref>PMID:11943159</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1gq8" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
| - | *[[Methylesterase|Methylesterase]] | + | *[[Methylesterase 3D structures|Methylesterase 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Daucus carota]] | [[Category: Daucus carota]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Eklund | + | [[Category: Eklund H]] |
| - | [[Category: El-Ahmad | + | [[Category: El-Ahmad M]] |
| - | [[Category: Friemann | + | [[Category: Friemann R]] |
| - | [[Category: Johansson | + | [[Category: Johansson K]] |
| - | [[Category: Jornvall | + | [[Category: Jornvall H]] |
| - | [[Category: Markovic | + | [[Category: Markovic O]] |
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Current revision
Pectin methylesterase from Carrot
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