1n3y

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[[Image:1n3y.jpg|left|200px]]<br /><applet load="1n3y" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n3y, resolution 1.65&Aring;" />
 
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'''Crystal structure of the alpha-X beta2 integrin I domain'''<br />
 
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==Overview==
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==Crystal structure of the alpha-X beta2 integrin I domain==
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The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response. We now report the structure of the alpha X I domain resolved at 1.65 A by x-ray crystallography. To analyze structural requirements for ligand binding we made a mutation in the alpha X I domain C-terminal helix, which increased the affinity for iC3b approximately 200-fold to 2.4 microM compared with the wild-type domain affinity of approximately 400 microM. Gel permeation chromatography supported a conformational change between the wild-type and mutated domains. Conservation of allosteric regulation in the alpha X I domain points to the functional importance of this phenomenon.
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<StructureSection load='1n3y' size='340' side='right'caption='[[1n3y]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n3y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3y OCA], [https://pdbe.org/1n3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3y RCSB], [https://www.ebi.ac.uk/pdbsum/1n3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ITAX_HUMAN ITAX_HUMAN] Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3y ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3Y OCA].
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*[[Integrin 3D structures|Integrin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure and allosteric regulation of the alpha X beta 2 integrin I domain., Vorup-Jensen T, Ostermeier C, Shimaoka M, Hommel U, Springer TA, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1873-8. Epub 2003 Jan 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12554829 12554829]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hommel, U.]]
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[[Category: Hommel U]]
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[[Category: Ostermeier, C.]]
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[[Category: Ostermeier C]]
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[[Category: Shimaoka, M.]]
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[[Category: Shimaoka M]]
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[[Category: Springer, T A.]]
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[[Category: Springer TA]]
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[[Category: Vorup-Jensen, T.]]
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[[Category: Vorup-Jensen T]]
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[[Category: alpha/beta rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:58 2008''
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Current revision

Crystal structure of the alpha-X beta2 integrin I domain

PDB ID 1n3y

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