3oto

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[[Image:3oto.png|left|200px]]
 
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{{STRUCTURE_3oto| PDB=3oto | SCENE= }}
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==Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)==
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<StructureSection load='3oto' size='340' side='right'caption='[[3oto]], [[Resolution|resolution]] 3.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3oto]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oto OCA], [https://pdbe.org/3oto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oto RCSB], [https://www.ebi.ac.uk/pdbsum/3oto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oto ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/3oto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3oto ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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All organisms incorporate post-transcriptional modifications into ribosomal RNA, influencing ribosome assembly and function in ways that are poorly understood. The most highly conserved modification is the dimethylation of two adenosines near the 3' end of the small subunit rRNA. Lack of these methylations due to deficiency in the KsgA methyltransferase stimulates translational errors during both the initiation and elongation phases of protein synthesis and confers resistance to the antibiotic kasugamycin. Here, we present the X-ray crystal structure of the Thermus thermophilus 30S ribosomal subunit lacking these dimethylations. Our data indicate that the KsgA-directed methylations facilitate structural rearrangements in order to establish a functionally optimum subunit conformation during the final stages of ribosome assembly.
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===Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)===
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Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function.,Demirci H, Murphy F 4th, Belardinelli R, Kelley AC, Ramakrishnan V, Gregory ST, Dahlberg AE, Jogl G RNA. 2010 Oct 20. PMID:20962038<ref>PMID:20962038</ref>
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{{ABSTRACT_PUBMED_20962038}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3oto" style="background-color:#fffaf0;"></div>
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[[3oto]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10|Ribosomal protein S10]]
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosomal protein S11|Ribosomal protein S11]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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== References ==
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*[[Ribosomal protein S13|Ribosomal protein S13]]
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<references/>
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*[[Ribosomal protein S14|Ribosomal protein S14]]
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__TOC__
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*[[Ribosomal protein S15|Ribosomal protein S15]]
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</StructureSection>
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*[[Ribosomal protein S16|Ribosomal protein S16]]
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[[Category: Large Structures]]
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*[[Ribosomal protein S17|Ribosomal protein S17]]
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[[Category: Thermus thermophilus HB8]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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[[Category: Belardinelli R]]
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*[[Ribosomal protein S19|Ribosomal protein S19]]
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[[Category: Dahlberg AE]]
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*[[Ribosomal protein S2|Ribosomal protein S2]]
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[[Category: Demirci H]]
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*[[Ribosomal protein S20|Ribosomal protein S20]]
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[[Category: Gregory ST]]
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*[[Ribosomal protein S3|Ribosomal protein S3]]
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[[Category: Jogl G]]
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*[[Ribosomal protein S4|Ribosomal protein S4]]
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[[Category: Kelley AC]]
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*[[Ribosomal protein S5|Ribosomal protein S5]]
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[[Category: Murphy IV F]]
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*[[Ribosomal protein S6|Ribosomal protein S6]]
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[[Category: Ramakrishnan V]]
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*[[Ribosomal protein S7|Ribosomal protein S7]]
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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*[[Ribosomal protein S9|Ribosomal protein S9]]
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*[[Ribosomal protein THX|Ribosomal protein THX]]
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==Reference==
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<ref group="xtra">PMID:020962038</ref><references group="xtra"/>
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[[Category: Thermus thermophilus]]
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[[Category: Belardinelli, R.]]
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[[Category: Dahlberg, A E.]]
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[[Category: Demirci, H.]]
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[[Category: Gregory, S T.]]
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[[Category: IV, F Murphy.]]
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[[Category: Jogl, G.]]
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[[Category: Kelley, A C.]]
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[[Category: Ramakrishnan, V.]]
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[[Category: 30s ribosomal subunit]]
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[[Category: Antibiotic resistance]]
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[[Category: Decoding]]
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[[Category: Decoding of genetic code]]
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[[Category: Ksga knock-out]]
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[[Category: Mrna]]
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[[Category: Post transcriptional rrna modification]]
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[[Category: Ribosome]]
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[[Category: Trna]]
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Current revision

Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)

PDB ID 3oto

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